砷胁迫对药用植物黄芪中抗氧化酶活性的影响  

Effects of Arsenic Stress on the Activities of Antioxidant Enzymes in Medicinal Plant Astragalus membranaceus(Fisch.) Bge. var. mongholicus(Bge.) Hsiao

作　　者：姜志艳[1] 王建英[1] 徐彩荣[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头014010

出　　处：《安徽农业科学》2011年第14期8279-8281,共3页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自然科学基金(2010MS0510)

摘　　要：[目的]探讨砷胁迫对药用植物黄芪中抗氧化酶活性的影响,以期为揭示砷毒害植物机理提供理论依据。[方法]以内蒙药用植物黄芪为材料,分别采用高锰酸钾滴定法、愈创木酚法、邻苯三酚自氧化法以及硫代巴比妥酸法研究不同浓度砷胁迫下对药用植物黄芪中过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性影响以和丙二醛(MDA)的浓度变化。[结果]随着砷浓度升高,黄芪叶片中这3种酶的活性能够维持,特别是POD活性显著增加。从时间动态看,随处理时间的延长,黄芪叶片中的CAT活性先升高再降低,POD活性增加,SOD活性变化不显著;MDA含量先升后降,第8天达到最大值。[结论]该研究可为中药材重金属含量控制和药理活性鉴定提供理论依据。[Objective]To discuss the effects of arsenic stress on the activities of antioxidant enzymes in medicinal plant Astragalus membranaceus（Fisch.） Bge.var.mongholicus（Bge.） Hsiao,so as to provide a theoretical basis for revealing the arsenic toxicity mechanism on plants.[Method] Using Astragalus membranaceus（Fisch.） Bge.var.mongholicus（Bge.） Hsiao as tested materials,potassium permanganate titration,guaiacol method,pyrogallol autooxidation methods and thiobarbituric acid method were adopted to study effects of arsenic stress on catalase（CAT）,peroxidase（POD）,superoxide dismutase（SOD） activities and malondialdehyde（MDA） concentrations.[Results] With the increase in concentration of arsenic,the activities of CAT,PCD and SOD in Astragalus membranaceus（Fisch.） Bge.var.mongholicus（Bge.）Hsiao could be maintained,especially POD.From the view point of dynamic time,CAT activity increased firstly then declined,POD activity increased and SOD activity changed little along with the increasing treatment time.Besides,MDA content increased firstly and then decreased,and reached the maximum on the 8th day.[Conclusions] The study could provide a theoretical basis for control of heavy metals in traditional Chinese medicine and identification of pharmacological activities.

关 键 词：蒙古黄芪(Astragalus membranaceus(Fisch.)Bge. VAR. mongholicus(Bge.)Hsiao) 砷胁迫 药用植物 抗氧化酶 酶活 

分 类 号：S567[农业科学—中草药栽培]