肺癌K-ras基因突变的二步PCR检测及其临床应用  

Two step PCR Detection of K ras Oncogene Mutation in Lung Cancer:Application to Clinic Diagnosis

作　　者：张新[1] 徐松涛 金建军[2] 周康[2] 刘康达[2] 李菁[1] 贾友民 

机构地区：[1]上海医科大学中山医院肺科,上海200032 [2]上海医科大学中山医院,上海200032

出　　处：《上海医科大学学报》1999年第5期329-332,共4页Journal of Fudan University(Medical Science)

基　　金：卫生部科研基金!资助 (96 - 1 - 1 62 )

摘　　要：目的　探讨高敏感性Kras基因点突变检测方法在肺癌临床的应用价值。方法　应用二步(巢式)聚合酶链反应结合限制性片断多态性分析方法(PCRRFLP)检测临床肺癌标本Kras基因第12密码子点突变。结果　二步PCRRFLP法检测Kras点突变的敏感性较一步法提高约64倍。55例肺癌石蜡包埋标本中,Kras点突变检出率为47.3%;对另67例纤支镜标本进行基因检测,以Kras突变进行基因诊断的敏感性为70.9%,特异性为91.7%。结论　二步PCRRFLP法对临床肺癌标本Kras点突变检出率较高。To evaluate the clinical significance of high sensitive assay to detect K ras gene point mutation in lung cancer. Methods The two step polymerase chain reaction combined with restriction fragment length polymorphism(PCR*.RFLP) assay was used to detect K ras codon mutation in clinical lung cancer specimen. Results The sensitivity of two step PCR*.RFLP was proved to be about 64 times of that of one step PCR.Fifty five resected lung cancer specimens embedded in paraffin were studied,and K ras mutation in 47.3% specimens was found.Sixty seven lung cancer specimens obtained from bronchoscope examination were studied using K ras mutation detection,the sensitivity of gene diagnosis was 70.9% and the specificity was 91.7%. Results The sensitivity of two step PCR*.RFLP was proved to be about 64 times of that of one step PCR.Fifty five resected lung cancer specimens embedded in paraffin were studied,and K ras mutation in 47.3% specimens was found.Sixty seven lung cancer specimens obtained from bronchoscope examination were studied using K ras mutation detection,the sensitivity of gene diagnosis was 70.9% and the specificity was 91.7%. Conclusions The two step PCR*.RFLP is helpful to diagnoses lung cancer because of its high sensitivity.

关 键 词：肺癌 K-ras癌基因 聚合酶链反应 辅助诊断 

分 类 号：R734.204[医药卫生—肿瘤]