阻塞性黄疸患者外周血淋巴细胞蛋白激酶C活性变化及意义  

Protein kinase C activities in peripheral blood lymphocytes in patients of obstructive jaundice

作　　者：王剑明[1] 邹蒨[1] 邹声泉[1] 

机构地区：[1]同济医科大学附属同济医院外科,武汉430030

出　　处：《中华普通外科杂志》1999年第6期443-445,共3页Chinese Journal of General Surgery

摘　　要：目的　研究蛋白激酶Ｃ（ｐｒｏｔｅｉｎ ｋｉｎａｓｅ Ｃ，ＰＫＣ）信息通道在阻塞性黄疸机体发病机理中的作用。 方法　从５１ 例阻塞性黄疸患者和１６ 例正常对照者的外周静脉血中分离和纯化淋巴细胞胞浆及胞膜部分蛋白激酶Ｃ，然后采用同位素γ３２ＰＡＴＰ催化活性测定法检测它们的活性。 结果　（１）阻塞性黄疸患者外周血淋巴细胞蛋白激酶Ｃ的总活性较正常健康人明显增强（ Ｐ＜ ０－０１） ，且胞膜部分蛋白激酶Ｃ活性占总活性的百分值较正常组也明显升高（ Ｐ＜ ０－０５）。（２）阻塞性黄疸患者淋巴细胞蛋白激酶Ｃ的活性与血清黄疸的程度（ＴＢＩＬ） 显著正相关（ｒ＝ ０－６７，Ｐ＜ ０－０１），与血清可溶性白细胞介素２ 受体（ｓｏｌｕｔｅｉｎｔｅｒｌｅｕｋｉｎ２，ｓＩＬ２Ｒ） 浓度显著正相关（ｒ＝ ０－５８ ，Ｐ＜ ０－０１）。 结论　蛋白激酶Ｃ信息通道的激活与黄疸程度有关，且可能参与了阻塞性黄疸时Ｔ淋巴细胞的活化。Objective To study the roles of protein kinase C (PKC) signal pathway in the pathogenesis of patients with obstructive jaundice. Methods PKC from cytosolic and membrane fractions of peripheral blood lymphocytes (PBL) was isolated and purified, its activities were determined by radioactive isotope γ 32 P ATP catalyzing assay. Results Fifty one patients with abstructive jaundice and sixteen normal controls were enrolled in this study. (1) The total PKC activities in PBL in patients with obstructive jaundice siginificantly increased as compared to those in normal subjects ( P <0 01). Moreover, the membrane PKC activities and their percentages of the total PKC activities were higher in patients with obstructive jaundice than in normal subjects ( P <0 05).(2) The total PKC activities in PBL was in positive correlation with the levels of solute IL 2 receptor (sIL 2R) ( r =0 67, P <0 01) and the degree of jaundice (T BIL) ( r =0 58, P <0 01) in serum. Conclusions The activities of PKC signal pathway related with the degree of jaurdice,it may be implicated in the activation of T lymphocytes. PKC signal pathway may be important in the immune regulation and the assessment of the condition in patients of obstructive jaundice.

关 键 词：胆汁郁积 蛋白激酶 阻塞性黄疸 淋巴细胞 外周血 

分 类 号：R575.02[医药卫生—消化系统]