大鼠急性牙髓炎造成中枢致敏时三叉神经脊束核极间亚核和孤束核c-fos蛋白的时程变化  

The change of c-fos protein in Vi and NTS under hyperalgesia induced by LPS-derived acute pulpitis

作　　者：顾斌[1] 刘洪臣[2] 马军利[3] 郭宏[1] 

机构地区：[1]解放军总医院口腔修复科,主治医师北京100853 [2]解放军总医院口腔医学中心,主任主任医师教授北京100853 [3]解放军总医院口腔颌面外科,主治医师北京100853

出　　处：《中华老年口腔医学杂志》2011年第1期12-14,共3页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金(30772450);北京市自然科学基金项目(7082086)

摘　　要：目的:研究大鼠急性牙髓炎造成中枢致敏时三叉神经脊束核极间亚核(interpolar part of spinaltrigeminal nucleus,Vi)和孤束核(nucleus tractus solitarius,NTS)内c-fos蛋白的变化情况。方法:建立大鼠急性牙髓炎模型,利用免疫组化实验检测不同时间点Vi核和NTS核c-fos蛋白的变化情况,采用单因素方差分析比较不同时间点的变化情况。结果:封药后12h至24h,Vi核c-fos阳性神经元表达数目明显增多;而双侧NTS内的c-fos阳性神经元数目在6h时最多,随后逐渐减少。结论:延髓内c-fos蛋白的变化时程与牙髓炎进展密切相关。Objective： To study the change of c-fos protein in Vi and NTS under hyperalgesia induced by LPS-derived acute pulpitis. Methods： A rat acute pulpitis model was induced, the express of e-los protein in Vi and NTS was screened by the means of immunoblot and the results were analysis by ANOVA. Result： The express of c-fos protein in Vi enhanced apparently at the time of 12h to 24h, The number of c-los positive neuron in NTS increased significantly till 6h after operation and then decreased gradually, but was sill higher than control group. Conclusion： The.expression of c-fos protein in medulla correlated closely with the development of acute pulpitis.

关 键 词：致敏 疼痛 C-FOS蛋白 

分 类 号：R781.31[医药卫生—口腔医学]