消痰散结方对人胃癌MNK-45荷瘤鼠机体HK-Ⅱ表达的影响  

Effects of Eliminating Phlegm Recipe on HK-Ⅱ Expression of MNK-45 Human Gastric Tumor-bearing Mice

作　　者：魏振[1] 魏品康[1] 巨大维[1] 张慈安[1] 周伟[1] 

机构地区：[1]第二军医大学长征医院中医科,上海200003

出　　处：《现代生物医学进展》2011年第10期1841-1843,1837,共4页Progress in Modern Biomedicine

摘　　要：目的:观察消痰散结方对人胃癌MNK-45荷瘤鼠糖酵解相关蛋白己糖激酶-Ⅱ(HK-Ⅱ)表达的影响,探讨该方抗肿瘤机理。方法:30只小鼠随机分为生理盐水组、消痰散结方组和希罗达组,每组10只。建立人胃癌MNK-45皮下移植瘤模型。灌胃治疗六周后,称取各组瘤质量,计算抑瘤率和瘤体积。酶联免疫吸附测定法检测荷瘤鼠血清中HK-Ⅱ的水平;免疫组化方法检测瘤组织中HK-Ⅱ蛋白的表达;结果:消痰散结方可明显抑制裸鼠人胃癌MNK-45皮下移植瘤生长,下调荷瘤鼠血清及瘤组织中HK-Ⅱ表达水平。结论:降低荷瘤鼠血清和组织中HK-Ⅱ的表达,抑制荷瘤鼠机体糖代谢过程可能是该方抗肿瘤作用的机制之一。To explore the antitumor effect of eliminating phlegm recipe on MNK-45 human gastric tumor-bearing mice. Methods： Thirty Kunming mice were randomly divided into normal saline （NS） group, Xiaotan Sanjie Recipe group and Xeloda group, ten for ench.Tumor-bearing models were established by injecting human gastric cancer MKN-45 injected into the subcutaneous site of BALB/c/-nu/nu mice. The mice were administered with medicine for six weeks. Weight of tumor xenografts were measured. tumor inhibiton rate and tumor volume were calculated. The expression of HK- Ⅱ was tested by enzyme-linked immunosorbent assay and immunohistochemistry. Results： Xiaotan sanjie Recipe group significantly inhibits the growth of the transplanted tumor and decrease The expression of ilK- Ⅱ in blood and tumor tissue. Conclusion： Xiaotan sanjie Recipe Could decrease the expression of ilK-Ⅱ in blood and tumor tissue,It may be one of the mechanisms of its in inhibiting the tumor growth.

关 键 词：消痰散结方 肿瘤 糖酵解 小鼠 

分 类 号：R735.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]