姜黄素对糖尿病大鼠肾组织AP-1的DNA结合活性升高的抑制作用  

Inhibition effect of curcumin on AP-1 DNA-binding activity increased in the kidney of experimental diabetic rats

作　　者：郝存勖[1] 苏彦君[1] 王青菊[2] 王丽[1] 李华[1] 杜鸿晧 杜九生 

机构地区：[1]石家庄市第二医院,河北050051 [2]石家庄市医保中心

出　　处：《医学动物防制》2011年第5期417-418,421,共3页Journal of Medical Pest Control

摘　　要：目的探讨姜黄素对糖尿病大鼠肾组织转录因子活化蛋白-1(activator protein-1,AP-1)的DNA结合活性升高的抑制作用。方法大鼠随机分为正常对照组(A组)、糖尿病组(B组)及姜黄素治疗组(C组),采用腹腔单剂量注射链脲佐菌素(streptozotocin,STZ)65mg/kg建立糖尿病大鼠模型。治疗组给予姜黄素30mg/kg.d腹腔注射。实验第4周各组分别宰杀5只大鼠,取部分右肾皮质,采用电泳迁移率改变分析(elec-trophoresis mobility shift assay,EMSA)检测姜黄素对糖尿病大鼠肾组织AP-1的DNA结合活性的影响,并观察肾组织的病理变化。结果糖尿病大鼠肾组织AP-1的DNA结合活性明显升高,姜黄素对其有显著抑制作用,并减轻糖尿病大鼠肾脏病变。结论姜黄素通过抑制糖尿病大鼠肾组织AP-1的DNA结合活性升高而减轻糖尿病大鼠肾脏病变。Objective To study the inhibition effect of curcumin on DNA-binding activity increased of AP-1 in the kidney of experimental diabetic rats.Method Rats were randomly divided into normal control group（group A）,diabetic group（group B）,and treatment group（group C）.Diabetes models were induced by streptozocin（STZ 65mg/kg） intraperitoneal injection,treatment group was given curcumin intraperitoneal injection（30mg/kg·d）.Five rats were killed in each group at the 4th week,took the renal cortex of right kidney,and detected DNA-binding activity of AP-1 by electrophoresis mobility shift assay（EMSA）,and observed the pathological changes of kidney.Results The DNA-binding activity of AP-1 in diabetic group was significantly increased.Curcumin can inhibition the activity of AP-1 and lessen renal lesions of diabetic rats.Conclusions Curcumin can decrease the damage of diabetic rats＇ kidney through inhibiting DNA-binding activity of AP-1.

关 键 词：姜黄素 电泳迁移率改变分析 AP-1 糖尿病肾病 

分 类 号：R-332[医药卫生]