重组腺病毒介导的野生型p53基因对淋巴瘤细胞放疗敏感性的影响  

Effect of Recombinant Adenovirus-mediated Wild-type p53 Gene Transfection on Lymphoma Cells

作　　者：李栋庆 范我[2] 俞泽阳[3] 

机构地区：[1]常州第二人民医院,江苏常州213003 [2]苏州大学核医学教研室,江苏苏州215123 [3]苏州大学附属第二医院,江苏苏州215004

出　　处：《中国医药指南》2011年第15期196-198,共3页Guide of China Medicine

摘　　要：目的观察重组腺病毒介导的野生型p53基因药物转染及联合辐射作用于淋巴瘤细胞后的表达状况及对其产生的杀伤效应。方法用MT(Tmethyl thiazolyl tetrazolium)法测定不同药物浓度、作用不同时间、不同辐射剂量及药物联合辐射对于raji和daudi细胞的生长抑制率;分别针对raji和daudi细胞设置对照组、加药组、照射组、联合组,进行蛋白提取,Western-Blotting法测定p53蛋白的表达,将图像进行比对分析。并用SPSS14.0软件对于上述数据进行分析。结果 raji和daudi细胞的加药组的生长抑制率明显于高于对照组(P<0.05)。两种细胞照射组的生长抑制率则随照射剂量的增加而上升。daudi细胞联合组的生长抑制率在各个剂量点上与照射组相比均无显著差异(P>0.05)。实验组的p53蛋白表达量均比对照组稍多,但两种细胞的联合组和照射组的p53蛋白表达量无明显差异(P>0.05)。结论重组腺病毒介导的p53基因药物能够转染入淋巴细胞,并表达p53蛋白,其对于raji和daudi淋巴瘤细胞有一定的抑制作用。重组腺病毒介导的p53基因药物对于增加放疗效果基本没有帮助。Objective The purpose is to observe the expression of the P53 protein and its effects after the transfection of recombinant adenovirus-mediated wild-type p53 gene drug and the joint radiation on the lymphoma cell lines（raji and daudi）.Methods To determine the inhibition rates of Raji and daudi cells interfered with different concentrations of drug,different transfection time,different radiation dose and the joint radiotherapy treatment with MTT assay.Daudi and raji cells were divided into the control group,drug group,radiotherapy group and joint group.Western Blotting assay was used for detecting the protein after the extraction of each group and the images were scanned for analysis.Spss14.0 software was used for single-factor analysis of variance.Results The inhibition rates of the drug groups of raji and daudi cells were significantly higher than that of the control group（P0.05）.Inhibition rates increase with the radiation dose in radiotherapy groups of two cell lines.Inhibition rate of daudi cells in the joint group of each dose point was not significantly different from the radiotherapy group（P0.05）.P53 protein expressions of the both experimental groups showed slightly higher than that of the control group The protein expressions of the joint groups of raji cells and daudi cells have no significant difference with the radiotherapy group（P0.05）.Conclusion The adenovirus-mediated p53 gene drug can transfect the lymphoma cells,and express the P53 protein,the inhibitory effects for raji and daudi lymphoma cells are to a certain extent.Recombinant adenovirus-mediated p53 gene drug can＇t increase the effect of radiotherapy.

关 键 词：基因治疗 恶性淋巴瘤 基因 P53 

分 类 号：R733.4[医药卫生—肿瘤]