枯草芽孢杆菌ON-6菌株抑制荔枝炭疽菌活性物质的初步研究  

Antagonistic Ability against Lichi Colletrichum gloeosporiodes Penz of Crude Extract of Bacillus subtilis ON-6

作　　者：黄曦[1] 张荣灿[2] 王何健[3] 黄荣韶[4] 黄庶识[3] 

机构地区：[1]广西药用植物园,南宁530023 [2]广西科学院海洋环境监测中心,南宁530007 [3]广西科学院生物物理实验室,南宁530003 [4]广西大学农学院,南宁530007

出　　处：《中国农学通报》2011年第13期188-193,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"基于激光镊子拉曼系统和单细胞分析技术的杆菌孢子萌发机理的研究"(30860010);广西科学基金项目"枯草芽孢杆菌对龙眼;荔枝采后病害的生物防治机理研究"(桂科回0832006);广西科研院所基本科研业务费资助项目"北部湾海洋微生物抗菌/抗肿瘤活性物质的研究"(08YJ16WL01)

摘　　要：为了探索枯草芽孢杆菌(Bacillus subtilis)对广西重要的药食两用水果——荔枝采后病害的防治效果及其机制,在进行采集、分离、筛选和鉴定的基础上,对枯草芽孢杆菌ON-6菌株发酵液提取拮抗物质方法、拮抗物质的理化性质等方面进行了研究。对ON-6菌株发酵液采用浓盐酸沉淀法及硫酸铵沉淀法,得到的提取液均有抑菌活性,表明ON-6菌株能产生能蛋白类和脂肽类抗生物质。对该菌株的蛋白粗提液进行30～60℃处理30min,其活性保持不变,121℃处理20min,活性丧失不足原有的40%;作用活性pH在5.0～8.0范围内;对胰蛋白酶不敏感,对蛋白酶K和胃蛋白酶部分敏感;对紫外线不敏感。Bacillus subtilis is a kind of beneficial microbes in biological control and fruit preservation currently.In order to explore the biocontrol effect and mechanism of Bacillus subtilis to litchi postharvest pathogenic fungi in Guangxi,experiments had been carried out to study the method of antifungal substances extraction from fermentation liquid,the partial physical and chemical properties of antifungal substances in Bacillus subtilis strain based on collection,separation,screening and identification.Antifungal substances of strain ON-6 with hydrochloric acid and ammonium sulfate both had inhibitant activity,showed that strain ON-6 were able to produce the proteins and lipopeptide antibiotics simultaneously.The inhibitant activity of crude antifungal protein produced by strain ON-6 was also stable when it was treated with 30-60℃ for 30 minutes,but was depressed less than 40% of the original with 121℃ for 20 minutes.Antifungal substances were partially sensitive to trypsin,and insensitive to proteinase K,pepsin and UV.The inhibitant activity pH rang of antifungal substances was from 5.0 to 8.0.

关 键 词：枯草芽孢杆菌 荔枝炭疽菌 抑菌活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]