蟾酥注射液对免疫抑制小鼠免疫功能的调节作用  

Regulative Effects of Bufonis Parenteral on Immunosuppression in Mice

作　　者：郭维霄[1] 仲伟婷[1] 李文华[1] 杨小丰[1] 杨振国[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《中国农学通报》2011年第14期45-49,共5页Chinese Agricultural Science Bulletin

基　　金："十一五"国家科技支撑计划"新型动物药剂研制与应用"(2006BAD31B03)

摘　　要：为研究蟾酥注射液对免疫抑制小鼠免疫功能的调节作用,采用免疫药理学方法制造免疫低下的动物模型,测定脾脏重量并计算脾脏指数;ELISA方法检测IgG,IgG1,IgG2b抗体水平;MTT法检测脾淋巴细胞增殖反应;ELISA方法检测细胞因子(IFN-γ、IL-4、IL-10)水平。测定结果表明,蟾酥注射液能够提高免疫低下小鼠脾脏指数,提高抗体水平;在ConA或者LPS刺激下提高脾细胞增殖率,促进淋巴细胞分泌细胞因子,说明蟾酥注射液在一定剂量下能够缓解氟苯尼考引起的免疫抑制。In the present study, regulative effects of bufonis parenteral sloution on-induced immunosuppression in mice were evaluated. Animal model of fl orfenicol-induced immunosuppression was made. Slpeens were weighted and calculated weight index. OVA-specific IgG, IgG 1 , IgG 2b were measured by ELISA, splenocytes were harvested for determining lymphocyte proliferation and spleen lymphocyte secrete cytokines were measured by ELISA method. The results showed that bufonis parenteral solution could increase the weight and the level of antibody. Bufonis parenteral solution, treated with polyclonal T, B cell mitogen （Con A or LPS） in a certain dose range, enhanced the proliferation significantly. Bufonis parenteral solution also induces cytokines secretion in vivo. The results indicated that bufonis parenteral solution possessed the regulative effects on florfenicol-induced immunosuppression in mice.

关 键 词：氟苯尼考 蟾酥注射液 OVA 免疫抑制 细胞因子 

分 类 号：S8[农业科学—畜牧兽医]