病毒激活巨噬细胞产生的一氧化氮与细胞毒的关系  

The study of relationship between Nitric Oxide(NO) and the cytotoxic activies of peritoneal elicited macrophage(PEM) induced by Newcastle Disease Virus losota strain(NDV L)

作　　者：白莲花[1] 于春[1] 刘倩[1] 李娟[1] 鲍秋莉 陈乃玲[1] 

机构地区：[1]北京军区总医院全军肝病研究所

出　　处：《中国免疫学杂志》1999年第8期356-358,共3页Chinese Journal of Immunology

基　　金：全军"九五"攻关基金

摘　　要：目的：确定新城鸡瘟病毒Ｌｏｓｏｔａ系（ＮＤＶＬ）能否诱导小鼠腹腔巨噬细胞（ＰＥＭφ）产生一氧化氮（ＮＯ）以及该ＮＯ与小鼠ＰＥＭφ介导的细胞毒作用是否有依赖关系。方法：采用Ｇｒｉｅｓ、ＦＡＣＳ分析和噻唑蓝（ＭＴＴ）方法。结果：ＮＤＶＬ与ＰＥＭφ作用２ｈ后就可以稳定地吸附在ＰＥＭφ表面，吸附率达８０％左右；收集ＮＤＶＬ与ＰＥＭφ作用２４ｈ上清，发现有ＮＯ的释放，释放量与阳性对照组（ＢＣＧＬＰＳＰＥＭφ上清）的释放量接近；采用ＭＴＴ法测定ＮＤＶＬＰＥＭφ对Ｈ２２肝癌细胞、Ｐ８１５肥大细胞瘤、Ｒａｊｉ细胞和Ｋ５６２白血病细胞的细胞毒活性（效／靶比为２０∶１），发现ＮＤＶＬＰＥＭφ对这些靶细胞具有明显的杀伤活性，且该活性与ＮＤＶＬＰＥＭφ诱导ＮＯ之间有一定的依赖性。结论：新城鸡瘟病毒Ｌｏｓｏｔａ（ＮＤＶＬ）能诱导小鼠巨噬细胞产生和释放一氧化氮。Objective:Determine if NDV L can induce mouse PEMφ releasing Nitric Oxide (NO) and the relationship between the NO and the cytotoxic activity of PEMφ. Methods:Used Griess FACS and MTT analysis. Results:NDV L could absorb steadily on the surface of PEMφ after infecting PEMφ for 2 h and absorption ratio was about 80 percent. When detecting the supernatant liquid which NDV L infected PEMφ for 24 h found that the quantities of NO was almost equal to the positive control group (BCG LPS PEMφ); While using MTT assay also found that the cytotoxic activities of NDV L PEMφ against H 22 , P 815 , K 562 and Raji targets was much higher than the ones of control group and there were some dependent relationship between NO and the cytotoxic activities of PEMφ. Conclusion:Newcastle Disease Virus can induce macrophages producing Nitric Oxide (NO) and there was relationship between the NO and the cytotoxic activities of PEMφ.

关 键 词：一氧化氮 鸡瘟病毒 细胞毒活性 肿瘤 免疫学 

分 类 号：R730.3[医药卫生—肿瘤]