儿茶酚-O-甲基转移酶基因多态性在新疆伊犁地区人群中的分布及与食管鳞癌的关系  

Distribution of catechol-O-methyltransferase polymorphisms in Yili profecture population of Xin- jiang and its relationship with esophageal squamous cell carcinoma

作　　者：黄丛改[1] 刘涛[1] 吕国栋[1] 郑树涛[1] 伊力亚尔·夏合丁 卢晓梅 

机构地区：[1]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054 [2]新疆维吾尔自治区食管癌研究所

出　　处：《中华实验外科杂志》2011年第6期957-960,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30760223、30860097）;新疆医科大学第一附属医院科研奖励基金资助项目（2008-YFY-01）

摘　　要：目的探讨儿茶酚-O-甲基转移酶（COMT）基因Vall58Met多态性在新疆伊犁地区人群中的分布及与食管鳞癌（ESCC）的关系。方法研究对象共487例，包括169例ESCC，318例正常对照。采用病例一对照研究方法，以聚合酶链反应-限制性片段长度多态性方法（PCR—RFLP）分析COMT基因G／A（Val／Met）多态性。结果487例新疆伊犁地区受试者COMT基因型频率分布依次为GG型50．7％，GA型41．1％，AA型8．2％；等位基因G为71．3％，A为28．7％；分层分析显示，新疆伊犁地区年龄≤60岁的ESCC组与对照组之间差异有统计学意义（P〈0．05）；将年龄及性别校正后，ESCC组与对照组COMTVall58Met多态性于哈族、汉族差异均无统计学意义（P〉0．05）。结论COMT基因Vall58Met多态性可能不是新疆伊犁地区哈萨克族、汉族ESCC危险性的遗传标志。Objective To explore the distribution of Val158Met polymorphism in catechol-O-meth- yltransferase （COMT） gene and its genetic susceptibility for esophageal squamous cell carcinoma （ESCC） of Yili population. Methods A case-control study was designed with 487 samples from 169 ESCC patients and 318 controls. Genotypes were determined by polymerase chain reaction-restriction fragment length poly- morphism （PCR-RFLP） approaches. Results The frequencies of COMT Vall58Met polymorphism GG, GA and AA genotype were 50. 7% , 41.1% and 8.2% in all subjects, and the frequencies of allele were 71.3% （G） , 28. 7% （A）. Stratified analysis by less than 60 years old showed that there were statistically significant differences in both frequencies of COMT Val158Met polymorphism and allele between controls and ESCC group in Yili Prefecture, Xinjiang （P 〈 0. 05 ）. After adjusting age and gender, there was no difference in Val]58Met polymorphism in Kazakh and Han ethnics between ESCC group and control group （P 〉0. 05 ）. Conclusion There was no association of genetic polymorphisms in the COMT Vall58Met with risk of ESCC of Kazakh and Han ethnics of Yili Prefecture, Xinjiang.

关 键 词：食管鳞癌 儿茶酚-O-甲基转移酶 基因多态性 

分 类 号：R735.1[医药卫生—肿瘤]