蛋白免疫印迹法检测胃癌组织Cyr61和HIF-1α蛋白的表达及其临床意义  

Expression and clinical significance of Cyr61 protein and HIF-1α protein in the gastric cancer by western blot

作　　者：许之美[1] 司君利[1] 钮自宇[1] 王贺[2] 亓玉琴[1] 隋爱华[3] 

机构地区：[1]青岛大学医学院附属青岛市市立医院消化内一科,山东青岛266011 [2]青岛市传染病医院,山东青岛266000 [3]青岛大学附属医院临床医学研究所,山东青岛266003

出　　处：《中国现代医学杂志》2011年第14期1620-1624,共5页China Journal of Modern Medicine

摘　　要：目的侵袭转移是恶性肿瘤最重要的生物学行为之一,也是导致死亡率增加的主要原因,而肿瘤细胞发生转移的机制一直是研究的热点。研究旨在检测富含半胱氨酸61(Cyr61)蛋白和低氧诱导因子-1α(HIF-1α)蛋白在胃癌组织中的表达水平,探讨两者与胃癌侵袭转移的关系。方法将标本分成3组,分别为49例胃癌组、49例配对的癌旁组(距癌2 cm)和49例配对的正常组(距癌5 cm以上),用免疫组织化学和蛋白免疫印迹(Western blot)方法检测Cyr61和HIF-1α的蛋白表达水平,并分析两者与胃癌的临床病理特征的关系。结果免疫组织化学检测显示Cyr61在胃癌细胞的胞浆中表达,HIF-1α在胃癌细胞的胞浆/胞核中表达。进一步用Western blot检测结果显示,胃癌中Cyr61蛋白的表达水平(0.2767±0.02200)高于癌旁组织(0.2145±0.02209)和正常组织(0.1055±0.02082),癌旁组织高于正常组织(均P<0.01);胃癌中HIF-1α蛋白的表达水平(0.2663±0.02682)高于癌旁组织(0.2120±0.04087)和正常组织(0.1008±0.03867)(均P<0.01),癌旁组织高于正常组织(P<0.01)。Cyr61和HIF-1α蛋白表达的增强与肿瘤的分化程度、淋巴转移及TNM分期相关(均P<0.05)。SPERMAN等级相关分析证明了Cyr61蛋白和HIF-1α蛋白的表达呈明显正相关(r=0.411,P<0.05)。结论 Cyr61蛋白和HIF-1α蛋白在胃癌组织中呈高表达,可能与胃癌的侵袭转移有关。联合检测Cyr61蛋白和HIF-1α蛋白对指导胃癌临床及判断预后有重要价值。【Objective】 Invasion and metastasis are one of the commonest biological behaviour of malignant tumor and the main causes of increasing mortality.The mechanism of invasion has become a hot research focus.The expression of Cyr61 and HIF-1α protein in gastric neoplasms was detected,and also their correlation with invasion and metastasis was investigated in this research.【Methods】 The methods of Inmuohistochemistry and Western blot were used to detect the expression of Cyr61 and HIF-1α in specimens from 49 patients with gastric cancer,49 paired adjacent gastric neoplasms（2 cm from the carcinoma）,and 49 surgical margins of paired normal gastric tissues（5 cm from the carcinoma）,to analyze their correlation with clinicopathologic characteristics of gastric neoplasm.【Results】 The method of Inmuohistochemistry indicated Cyr61 expressed in the cytoplasm of gastric cancer;HIF-1α expressed in the both of cytoplasm and nucleus.And the results of Western blot were as follows： The expression of Cyr61 protein was higher in gastric neoplasms（0.2767±0.02200） than that in the paired adjacent gastric neoplasms（0.2145±0.02209） and normal gastric tissue（0.1055±0.02082）（all P 0.01）.The expression of HIF-1α protein was higher in gastric cancer（0.2663±0.02682） than that in the paired adjacent gastric neoplasms（0.2120±0.04087） and normal gastric tissue（0.1008±0.03867）（all P 0.01）.The increase expression of Cyr61 and HIF-1α were closely associated with the degree of differentiation,lymph node metastasis,and TNM staging of gastric cancer（all P 0.05） Spearman rank correlation analysis demonstrated a significant correlation between Cyr61 and HIF-1α（r =0.411,P 0.05）.【Conclusion】 The expression of Cyr61 and HIF-1α protein were significantly highest in gastric cancer and may promote the invasion and metastasis of gastric cancer.The determination of Cyr61 and HIF-1α may be of value in the treatment as well as in the prognosis of patients with gastric cancer.

关 键 词：胃肿瘤 富含半胱氨酸61 低氧诱导因子-1Α 免疫组织化学 蛋白免疫印迹法 

分 类 号：R735.2[医药卫生—肿瘤]