三种土壤中土霉素浸提方法的比较研究  

Compare of different oxytetracycline extraction methods in the three soils

作　　者：余强[1] 鲍艳宇[1] 李艳梅[1] 周启星[1,2] 刘雨霞[1] 

机构地区：[1]南开大学环境科学与工程学院,教育部环境污染过程与基准重点实验室,天津市城市生态环境修复与污染防治重点实验室,天津300071 [2]中国科学院沈阳应用生态研究所,中国科学院陆地生态过程重点实验室,辽宁沈阳110016

出　　处：《中国环境科学》2011年第6期951-957,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(40901259)

摘　　要：以棕壤、褐土和红壤为研究对象,比较了不同浸提方法,不同浸提剂对土壤中土霉素的浸提效率.结果表明,在所用的3种方法中,采用振荡浸提方法,以及Na2EDTA-McIlvaine缓冲液对土霉素具有稳定、高效的浸提回收率.土霉素形态初步分析连续浸提步骤为:以H2O、0.1mol/L CaCl2、和Na2EDTA-McIlvaine缓冲液为浸提剂依次浸提可分别得到水溶态、可交换态、吸附态和固定态土霉素.3种土壤中土霉素形态呈现吸附态>可交换态>水溶态的规律.Recoveries of oxytetracycline extracted were discussed with different extractants and extraction processes in the brown,cinnamon,and red soil.Stable and efficient extracted recovery could be achieved by using Na2EDTA-McIlvaine as a extractant in the shake flask extraction.In addition,a sequential extraction procedure was developed to study the chemical speciation of oxytetracycline in the soils.The soils were extracted by H2O,0.1mol/L CaCl2,and Na2EDTA-McIlvaine in order.As a result,water-soluble,exchangeable,and adsorbed fractions were obtained.Moreover,it could conclude that the orders of amount of those fractions are as follows： adsorbed exchangeable water-soluble fractions in the three tested soils.

关 键 词：土霉素 浸提方法 土壤 化学形态 

分 类 号：X53[环境科学与工程—环境工程]