一株非发酵反硝化聚磷菌的筛选及其代谢模式研究  

Screening and metabolism of non-fermentive denitrifying phosphorus accumulating bacteria(DPB)

作　　者：刘晖[1,2,3] 孙彦富[2] 贾晓珊[1] 李军[3] 周康群[2] 熊蒂程[3] 区向东 

机构地区：[1]中山大学环境科学与工程学院,广东广州510275 [2]仲恺农业工程学院环境科学与工程系,广东广州510225 [3]北京工业大学建工学院,北京100124

出　　处：《中国环境科学》2011年第6期958-964,共7页China Environmental Science

基　　金：国家重大科技专项"水体污染控制与治理"(2008ZX07211-003);国家自然科学基金资助项目(51078008);广东自然科学基金资助项目(06022869;07003251);广东省教育厅项目(LYM08067)

摘　　要：利用活性污泥与生物膜复合系统富集反硝化聚磷菌(DPB)并进行了功能菌的筛选及反硝化聚磷实验,考察了乙酸、PO43--P和NO3--N、PHB和糖原的变化,分析了厌氧和缺氧代谢模式以及PHB在此过程中所起的作用.结果表明,分离到的YB菌株与脱氮副球菌属最相似,是一类非发酵的反硝化聚磷菌.厌氧阶段,YB菌株合成1mg的PHB释放0.11mg的P,作为非发酵菌,合成PHB所需要的能量ATP大部分来自聚磷酸盐的释放.缺氧阶段,YB菌株分解1mg的PHB聚集0.15mg的P,PHB分解产生的ATP多用于聚磷过程;与以前报道的聚磷菌在厌氧和缺氧条件下的代谢模式不同.厌氧阶段聚磷酸盐的释放将影响PHB的合成,进一步影响反硝化聚磷效果,因此认为应重点跟踪△P/△PHB和△NO3--N/△PHB,而不是△P/△COD、△NO3--N/△COD或者△P/△NO3--N.The activated sludge and bio-membrane system was used to enrich denitrifying phosphorus bacteria（DPB） for removing nitrogen and phosphorus simultaneously.A DPB strain was screened after enrichment and denitrifying phosphorus accumulating experiments were investigated.The change of HAC/phosphate/nitrate/PHB and glycogen were studied in order to analyze the anaerobic and anoxic metabolism and the function of PHB during the process.Strain YB was similar to Paracoccus denitrificans.It was a kind of non-fermentive DPB.Strain YB released 0.11mg P per 1mg PHB during anaerobic stage.As a kind of non-fermentive bacteria,the energy was mostly from the release of poly-phosphate.Strain YB accumulated 0.15 mg P per 1mg PHB during anoxic stage.ATP from the breakdown of PHB was mostly used to accumulate phosphate.The metabolism was different from that of reported phosphate accumulating organisms（PAO）.The release of poly-phosphate affected the synthesis of PHB and the impact of denitrifying and accumulating phosphate.It was very important to monitor △P/△PHB and △NO3--N /△PHB,not △P/△COD、△NO3--N /△COD or △P/△NO3--N.

关 键 词：反硝化聚磷菌 脱氮副球菌属 非发酵菌 代谢模式 

分 类 号：X172[环境科学与工程—环境科学]