青海弧菌Q67自体诱导物的检测及其对菌体发光的影响  

Detection of autoinducer in Vibrio qinghaiensis sp.-Q67 and its effect on luminescence

作　　者：叶姜瑜[1] 陈宇[2] 李书钺[1] 翟俊[1] 何强[1] 舒为群[3] 

机构地区：[1]重庆大学城市建设与环境工程学院,重庆400045 [2]西南大学生命科学学院,重庆400715 [3]第二军医大学军事预防医学院,重庆400038

出　　处：《微生物学通报》2011年第6期903-907,共5页Microbiology China

基　　金：国家重大水专项资助项目(No.2008ZX07315-003-02)

摘　　要：青海弧菌Q67是一种我国新近确定的淡水发光细菌,其发光强度随毒物浓度改变的特性使其可作为水质检测的指示菌株。利用生物检测菌株快速检测、C18反相薄层层析和β-半乳糖苷酶含量测定,证实了该菌存在LuxI-LuxR型群体感应系统,并产生N-酰基高丝氨酸内酯类自诱导物。进一步的实验表明,该信号分子活性随生长阶段有较大变化,其粗提物不仅能调控菌体的生物发光,对菌体生长繁殖也产生较大影响。Vibrio qinghaiensis sp.-Q67 was a newly identified luminescent bacterium in fresh wa-ter,its character of luminous intensity changed with toxic pollutant concentrations made it as an strain for water quality monitoring.Through bioassay strain JZA1 test,C18 reverse phase thin-layer chromatography and LacZ activity test,it was identified that Q67 had a LuxI-LuxR type Quo-rum-Sensing and produced N-acyl-homoserine lactone（AHL） as autoinducer.The other test results in-dicated that the content and activity of the signal molecular in Q67 changed with its growth phases.The crude extract from Q67 affected not only its luminescence but also its growth and reproduction.

关 键 词：青海弧菌 群体感应 AHLs类信号分子 发光强度 

分 类 号：X172[环境科学与工程—环境科学]