HPLC测定荷叶中碱盐的含量  

Study on determination of nuciferine salt by HPLC

作　　者：阎娜[1] 王炜华[1] 李俶[1] 刘成梅[1] 王一铭 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌济顺制药有限公司,江西南昌330008

出　　处：《食品与机械》2011年第3期71-72,87,共3页Food and Machinery

摘　　要：以荷叶为原料,建立一种荷叶碱盐含量的测定方法。运用高效液相色谱法测定,色谱柱为Hypersil ODS2(4.6mm×250mm,5μm),流动相为乙腈-磷酸盐缓冲溶液(0.05%磷酸-0.2%三乙胺-水)(25∶75,V/V),流速为1.0mL/min,检测波长为270nm。结果显示,荷叶碱盐在0.053～0.159mg/mL(R2=0.999 2)范围内具有良好的线性关系,平均回收率为96.4%,RSD为0.05%。该方法简便、准确,灵敏度高,重复性好,可用于荷叶碱盐成分的含量测定。To establish an HPLC method for the determination of nuciferine salt on lotus leaf.Methods： Analyses were carried out on Hypersil ODS2（4.6 mm×250 mm,5 μm）column with acetonitrile： 0.05% phosphoric acid-0.2% triethylamine-water（25∶75,V/V） as the mobile phase.The flow rate was 1.0 mL/min and the UV detection wavelength was 270 nm.Results indicated,Linearities of nuciferine salt was good in the ranges of 0.053～0.159 mg/mL（R2=0.999 2）.The average recovery was 96.4%,and RSD was 0.05%（n=6）.The method was simple,accurate,sensitivity and reproducible.The method can be used to the determination of nuciferine salt.

关 键 词：荷叶碱盐 含量测定 高效液相色谱 

分 类 号：S567.239[农业科学—中草药栽培]