砒霜对狼疮鼠脾Th细胞及分泌细胞因子的影响  

Effects of Arsenic Trioxide on Splenic Th Lymphocyte and its Cytokines Expressions in Lupus Mice

作　　者：夏晓茹[1] 孙莉[1] 陈培荣[1] 朱小春[1] 

机构地区：[1]温州医学院附属第一医院风湿免疫科,温州浙江325000

出　　处：《中华中医药学刊》2011年第6期1299-1302,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(M303606);温州市科技局资助项目(Y20060220;Y20090240)

摘　　要：目的:探讨砒霜(Arsen ic Trioxide,As2O3)对体外MRL/lpr狼疮小鼠脾Th细胞及分泌相关细胞因子IFN-γ和IL-10的调节作用。方法:通过免疫磁珠分别分选MRL/lpr和C57BL小鼠脾Th细胞,Th细胞随机分PHA组和空白组,采用酶联免疫吸附反应(ELISA)和逆转录-聚合酶链反应(RT-PCR)法比较MRL/lpr和C57BL小鼠Th细胞分泌IFN-γ和IL-10及其mRNA水平的差异,然后观察As2O3对MRL/lpr小鼠Th细胞分泌IFN-γ和IL-10及其mRNA水平的影响。细胞计数试剂盒(CCK-8)观察不同浓度(0.5、1.0、1.5、2.0μmol/L)及作用时间As2O3对Th细胞增殖的影响。结果:(1)与C57BL相比,MRL/lpr小鼠各组Th细胞分泌的IFN-γ和IL-10水平显著增高,1.0μmol/L的As2O3能明显抑制MRL/lpr小鼠各组上述因子的分泌;(2)PHA组的IL-10 mRNA和IFN-γmRNA水平在MRL/lpr小鼠中表达均高于C57BL小鼠,As2O3能抑制MRL/lpr小鼠Th细胞空白组和PHA组中IL-10mRNA和IFN-γmRNA的高表达;(3)除0.5μmol/L的As2O3能刺激Th细胞的增殖,≥1.0μmol/L的As2O3能抑制Th细胞的生长,但浓度增加对细胞存活率有明显的影响。结论:适当浓度的砒霜能有效下调Th细胞因子IL-10和IFN-g mRNA的异常分泌及而影响因子的表达,但并不产生过度的免疫抑制,并能下调MRL/lpr狼疮小鼠脾脏Th细胞的异常增殖而不影响其存活率,从而调节狼疮鼠的异常免疫功能,可能是砒霜治疗SLE的重要机制之一。Objective：To evaluate the effects of arsenic trioxide（As2O3） on the proliferation of Th lymphocytes,and the suppression on transcription and interpretation of interferon（IFN）-γ and interleukin（IL）-10 of MRL/lpr.Methods：Th cells in splenocytes were separated by Immunomagnetic beads from MRL/lpr and C57BL mice each respectively,the effect of As2O3 on Th cells proliferation was evaluated by Cell counting kit（CCK-8）;and then Th cells were divided randomly into two groups： PHA group and Blank group.The transcription and interpretation levels of IL-10 and IFN-γwere measured by ELISA and RT-PCR respectively,so the levels of IL-10 and IFN-γ of two groups by As2O3 were compared.Results：（1） Comparing with C57BL mice,the secretion of IFN-γ and IL-10 by Th cells in PHA group and Blank group of MRL/lpr mice were much higher;appropriate concentration of As2O3（1.0μmol/L） could inhibit secretion of these cytokines.（2） the IFN-γ and IL-10 mRNA levels on PHA group in MRL/lpr mice were much higher than that in C57BL,and As2O3 could suppress the transcriptions of IFN-γ and IL-10 mRNA in both PHA group and Blank group.（3）After a 24h＇s culture,As2O3（0.5μmol/L） do help the proliferation of Th cell,but≥ 1.0μmol/L could suppress the proliferation of Th cells,and survival rate is decreased when the dose of As2O3 increased.Conclusion：Appropriate concentration of As2O3 could depress the high level of interpretation and transcription of IFN-γ and IL-10 in lupus mice and regulate the cytokines near to normal level;and it could suppress the abnormal proliferation and activity of Th cell without affecting the survival rate.It indicated that As2O3 could regulate the immune dysfunction,so may turn to be a new drug for treating SLE.

关 键 词：系统性红斑狼疮 砒霜 TH细胞 IFN- γIL-10 

分 类 号：R285[医药卫生—中药学]