趋化因子受体5表达与乳腺癌临床病理特征及预后的相关性研究  

Analysis of the correlation between CCR5 expression and clinicopathological features and prognosis of breast cancer

作　　者：章阳[1] 孟凡云[2] 李文磊[1] 范海燕[2] 关众[1] 周长鑫[1] 

机构地区：[1]山东省聊城市人民医院乳腺甲状腺外科,山东聊城252000 [2]山东省聊城职业技术学院医学院,山东聊城252000

出　　处：《中国普通外科杂志》2011年第5期485-490,共6页China Journal of General Surgery

基　　金：山东省自然科学基金资助项目(Y2007C090)

摘　　要：目的探讨趋化因子受体5(CCR5)在乳腺癌病灶和腋窝转移淋巴结中的表达及其与临床病理意义。方法收集72例乳腺导管浸润癌及其腋窝淋巴结组织,50例乳腺纤维腺瘤组织,40例正常乳腺组织石蜡标本切片。采用免疫组化方法检测CCR5的表达情况;同时检测乳腺癌组织中C-erbB-2,p53,Ki-67,ER,PR的表达情况,并与临床病理资料进行统计学分析。结果 (1)乳腺癌组织中CCR5蛋白表达阳性率达84.72%(61/72),乳腺纤维腺瘤组织中表达阳性率较低(14%,7/50),正常乳腺组织中不表达CCR5;(2)乳腺癌CCR5表达与腋窝淋巴结转移呈正相关(χ2=4.982,P=0.026,r=0.305);(3)50例伴腋窝淋巴结转移的患者中,乳腺癌原发灶及转移淋巴结同时表达CCR5阳性者39例,具有较高的同源性;(4)乳腺癌CCR5表达与癌基因C-erbB-2之间呈低度正相关(P<0.05,r=0.291);(5)乳腺癌CCR5表达与患者年龄、绝经与否、肿瘤最大径、肿瘤分期等临床特征无关(均P>0.05);(6)乳腺癌CCR5表达与p53,Ki-67,ER,PR无关表达(均P>0.05)。结论 CCR5在乳腺癌的发生、发展及腋窝淋巴结转移方面起一定作用;CCR5可间接作为预测乳腺癌腋窝淋巴结转移及预后判断的指标之一。Objective To study the expression and clinicopathological relevance of chemokine receptor 5（CCR5） in breast cancer tissues and metastatic axillary lymph nodes.Methods The paraffin specimens of 72 cases of invasive ductal carcinoma（IDC） along with the invoved axillary lymph nodes,50 cases of breast fibroadenoma and 40 cases of normal mammary gland were collected.The expression of CCR5 protein in those specimens was detected by immunohistochemical staining.Meanwhile,the expressions of C-erbB-2,P53,Ki-67,ER and PR in the specimens of IDC were also measured with immunohistochemical method.Finally,the immunohistochemical results were statistically analyzed with regard to correlations with their clinicopathological data.Results（1） The positive expression of CCR5 in IDC was 84.72%（61/72）,breast fibroadenoma tissue presented low positive expression of CCR5（14%,7/50）,and no CCR5 expression was found in normal breast tissue.（2） The expression of CCR5 in IDC had a positive correlation with lymph node metastasis（χ2=4.982,P=0.026,r=0.305）.（3） Of the 50 cases with axillary lymph node metastasis,CCR5 was simultaneously expressed both in primary cancer and its invoved axillary lymph nodes in 39 cases,which showed a high homology.（4） There was a low positive correlation between CCR5 and breast cancer oncogene C-erbB-2（P0.05,r=0.291）.（5） CCR5 showed no relationship with age of onset,maximum diameter of tumor,mensis status or clinical stage（P0.05）.（6） CCR5 had no direct correlation with the expression of p53,Ki-67,ER,PR（P0.05）.Conclusions CCR5 plays an important role in occurrence and development as well as axillary lymph node metastasis of breast cancer.CCR5 may be one of the indirect indicators to predict axillary lymph node metastasis and prognosis of breast cancer.

关 键 词：乳腺肿瘤/病理学 趋化因子受体 C-ERBB-2 p53 KI-67 雌激体受体 孕激素受体 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]