survivin siRNA对裸鼠乳腺癌细胞移植瘤微血管生成的影响  

Effects of survivin siRNA on angiogenesis of human breast cancer xenografts in nude mice

作　　者：武彪[1] 刘正人[1] 李映良[1] 涂发妹[1] 刘东升[1] 吴冬娣[1] 吴玮斌[1] 

机构地区：[1]南昌大学第一附属医院普通外科,江西南昌330006

出　　处：《中国普通外科杂志》2011年第5期491-493,共3页China Journal of General Surgery

基　　金：江西省卫生厅科技计划资助项目(20081053)

摘　　要：目的探讨含survivin siRNA的PcDNA3-siRNA对裸鼠人乳腺癌MCF-7细胞移植瘤微血管生成的影响。方法制作裸鼠MCF-7细胞移植瘤模型,然后随机分为PcDNA3-siRNA处理组,PcDNA3处理组,空白对照组,成瘤后分别将PcDNA3-siRNA质粒,、PcDNA3空质粒,生理盐水注入裸鼠MCF-7移植瘤中。采用RT-PCR及Western blot半定量法检测各组移植瘤组织中survivin mRNA及survivin蛋白的表达;SP免疫组化法检测各组移植瘤中的微血管密度(MVD)。结果 PcDNA3-siRNA组survivin mRNA及survivin蛋白的表达量较PcDNA3组及空白对照组明显降低(P(0.05),且PcDNA3-siRNA组MVD明显低于PcDNA3组及空白对照组(P(0.05);PcDNA3组与空白对照组survivin mRNA,survivin蛋白及MVD值差异无统计学意义(P(0.05)。结论 PcDNA3-siRNA可抑制裸鼠MCF-7细胞移植瘤survivin的表达,而且对移植瘤的微血管生成具有明显的抑制作用。Objective To investigate the effects of survivin siRNA on angiogenesis of human breast cancer MCF-7 cell xenografts in nude mice.Methods The animal model of tumor xenograft was established by transplantation with MCF-7 cells into nude mice.The models were randomly assigned to 3 groups： PcDNA3-Survivin siRNA treatment,PcDNA treatment and control group,which were respectively injected with PcDNA3-siRNA plasmid,PcDNA3 empty plasmid or saline solution into the xenograft tumor after tumor formation in the mice.Survivin mRNA and protein levels were examined by RT-PCR and Western blot.Microvessel density was measured by immunohistochemistry staining.Results Survivin mRNA,protein levels and microvessel density in PcDNA3-Survivin siRNA treatment group were significantly decreased compared with PcDNA treatment and control group（P﹤0.05）;whereas no significant difference was noted between PcDNA treatment and control group（P﹥0.05）.Conclusions Survivin siRNA not only decreases survivin expression,but also inhibits angiogenesis in MCF-7 xenografts.

关 键 词：乳腺肿瘤/病理学 SURVIVIN SIRNA 裸鼠 血管生成 

分 类 号：R737.9[医药卫生—肿瘤]