多壁纳米碳圈对血小板聚集与止血功能的影响  

Effects of multiwall carbon naro-onions on platelet aggregation and hemostatic function

作　　者：杨桂丽[1] 杨洁[1] 章军[1] 徐玉英[1] 韦巧慧[1] 孙向珏[1] 顾晓敏[1] 张寅[1] 丁秋萍[1] 郑一凡[1] 杨军[1] 朱心强[1] 

机构地区：[1]浙江大学医学部公共卫生学院毒理学系,杭州310058

出　　处：《中华劳动卫生职业病杂志》2011年第5期321-323,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助（30771824,30800923）;中央高校基本科研业务费专项资金（2009QNA7021）

摘　　要：目的观察多壁纳米碳圈（MWCNOs）对大鼠血小板聚集和止血功能的影响。方法采用Born比浊法通过体内和体外试验测定MWCNOs对大鼠血小板聚集的影响。（1）体外试验：测定溶剂和不同剂量MWCNOs（0．2、2．0、20．0μg／ml）对大鼠血小板聚集的影响。（2）体内试验：将20只成年健康雄性sD大鼠随机分为溶剂组和MWCNOs组（2、4和8mg／kg），观察尾静脉注射染毒12h后MWCNOs对血小板数量、出血时间和凝血指标的影响。同时观察尾静脉注射4mg／kgMWCNOs和等体积溶剂作用不同时间（0．5、6和12h）后对血小板数量和血小板聚集的影响。结果MWCNOs对南二磷酸腺苷引起的血小板聚集有明显的抑制作用，并且随着剂量的增加，抑制作用增强。最高剂量组（20．0μg／m1）血小板聚集率（50．0％±6．9％）明显低于溶剂组（73．2％±4．3％），差异有统计学意义（P〈0．01）。体内试验结果同样观察到MWCNOs存在抑制血小板聚集的作用，2、4、8mg／kgMWCNOs组的血小板聚集率（分别为51．2％±4．5％、54．3％±7．8％、56．8％±3．0％）均低于溶剂组（64．3％±3．4％），但差异无统计学意义（P〉0．05）。未发现MWCNOs对大鼠血小板数量、出血时间和凝血指标有明显影响。结论在该研究条件下，MWCNOs对血小板聚集可能存在抑制作用，但并不影响机体的止血功能。Objective To observe the effects of multiwall carbon nano-onions （MWCNOs） on platelet aggregation and hemostatic function. Methods The platelet aggregation was determined with Born＇s method at different concentration of MWCNOs （0, 0.2, 2.0, 20.0 μg/ml） in vitro. Twenty male SD rats were randomly divided into 4 groups which were exposed to 0, 2, 4 and 8 mg/kg MWCNOs, respectively. Theu platelet count, platelet aggregation, activated partial thromboplastin time （APTT）, prothrombin time （PT）, thrombin time （TT）, bleeding time （BT） and platelet count （PC）were measured at 12 h after receiving tail intravenous injection of MWCNOs. The effeets of MWCNOs （4 mg/kg） on platelet aggregation and platelet count at different time points were observed. Results In vitro, MWCNOs exhibited the potent inhibitory effects on rat platelet aggregation caused by ADP in a concentration-dependent manner. The platelet aggregation in the highest dosage of 20.0 μg/ml group was 50.0±6.9% which was significantly lower than that （ 73.2%±4.3% ） in control group （P〈0.01）. In vivo, the highest inhibitory was up to 20.4%, but there was no significant difference, as compared with control group. MWCNOs did not affect the APTT, PT, TT, BT and PC. Conclusion Under this experimental condition, MWCNOs might inhibit platelet aggregation but not affeet hemostatic function.

关 键 词：纳米颗粒 血小板聚集 凝血酶原时间 凝血酶时间 

分 类 号：TB38[一般工业技术—材料科学与工程] R[医药卫生]