咖啡因对体外培养人毛囊及毛乳头细胞的影响  

Effects of caffeine on human hair follicles and the dermal papilla cells in vitro

作　　者：陈蕾[1] 关宁宁[1] 孙蔚凌[1] 刘嘉茵[1] 何绍衡[1] 范卫新[1] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029

出　　处：《临床皮肤科杂志》2011年第6期323-327,共5页Journal of Clinical Dermatology

基　　金：国家自然科学基金(30771947);江苏省自然科学基金(BK2007248);中华医学会欧莱雅中国人健康皮肤毛发研究基金(H2012091004)资助项目

摘　　要：目的:研究咖啡因对人毛囊及毛乳头细胞体外培养的影响。方法:选择不同浓度咖啡因作用于体外培养的人毛囊及毛乳头细胞.记录6 d内毛囊的生长速度和毛球部形态学改变,采用四甲基偶氮唑蓝(MTT)法测定毛乳头细胞增殖活性;流式细胞技术测定细胞凋亡:RT-PCR技术进行血管内皮生长因子(VEGF)、成纤维细胞生长因子(FGF)、雌激素受体(ER)、雄激素受体(AR)及5α-还原酶(SRD5A)的mRNA定量。结果:与对照组相比,0.005%咖啡因明显促进毛囊生长,0.000 5%咖啡因促进毛乳头细胞增殖、抑制凋亡,并促进毛发生长正性相关因子表达,抑制负性相关因子表达。结论:在有效浓度范围内,咖啡因可以促进人毛发生长,高浓度咖啡因抑制毛发生长。Objective： To investigate the effects of caffeine on the growth of human hair follicles and the dermal papilla cells （DPCs） in vitro. Methods： Different concentrations of caffeine were incubated with human hair follicles and the DPCs. The morphological changes in the hair bulbs and growth speed of the hair follicles were observed and recorded daily within 6 days. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis respectively. RT-PCR was also applied to analysis the expressions of VEGF, FGF, ER, AR and SRD5A. Results： Compared with the controls, 0.005% caffeine significantly stimulated the growth of hair follicles, and at this concentration it stimulated the proliferation and inhibited the apoptosis of human DPCs in vitro. RT-PCR re- suits showed that 0.000 5% caffeine significantly stimulated the expression of VEGF, FGF and ER and inhibited the expressions of AR and SRD5A. Conclusion： In the effective concentrations, caffeine can promote the growth of human hair. These effects may be concerned with its stimulating the expressions of VEGF, FGF and ER and inhibiting the expressions of AR and SRD5A.

关 键 词：咖啡因 毛囊 毛乳头细胞 

分 类 号：R971.7[医药卫生—药品] R322.995[医药卫生—药学]