氯通道在鼻咽癌细胞凋亡性细胞容积减小和细胞凋亡中的作用  

Effect of Chloride Channels on Apoptotic Volume Decrease and Apoptosis in Nasopharyngeal Carcinoma Cells

作　　者：潘廷才[1] 杨林杰[1] 刘善文[1] 李华荣[1] 朱林燕[1] 叶文才[2] 王立伟[1] 陈丽新[1] 

机构地区：[1]暨南大学医学院,510632 [2]暨南大学药学院

出　　处：《天津医药》2011年第6期487-489,共3页Tianjin Medical Journal

基　　金：国家自然科学基金项目(项目编号:30771106;30870567;30871267;90913020;U0932004)

摘　　要：目的:探讨氯通道在5-氟尿嘧啶(5-Fu)诱导的低分化鼻咽癌细胞CNE-2Z凋亡性细胞容积减小(AVD)和细胞凋亡中的作用。方法:培养CNE-2Z细胞后,分别用100μmol/L5-Fu(5-Fu组)、100μmol/L5-Fu+100μmol/L5-硝基-2-(3-苯丙胺)苯甲酸(5-Fu+NPPB组)处理细胞,采用活细胞影像系统实时拍摄细胞图像,检测细胞容积变化,Hochest33258荧光染色技术检测细胞凋亡并计算细胞凋亡率。结果:5-Fu处理使细胞皱缩,体积变小;5-Fu+NPPB处理后细胞体积变化不明显。在5个不同时间点,细胞受到5-Fu刺激后,标准化细胞容积(Vst)均小于对照组,5-Fu+NPPB组对细胞Vst的影响均小于5-Fu组,差异均有统计学意义。对照组细胞凋亡率(1.8±0.5)%,5-Fu处理使细胞凋亡率增加至(49.2±2.6)%,5-Fu+NPPB处理使细胞凋亡率降至(12.5±2.9)%。结论:抑制氯通道可显著拮抗5-Fu诱导的凋亡性细胞容积减小和细胞凋亡。Objective： To investigate the roles of chloride channels in apoptotic volume decrease （AVD） and apoptosis in nasopharyngeal carcinoma cells （CNE-2Z）. Methods： The CNE-2Z was cultured and treated with 100 μmol/L 5-Fu （5-Fu group）, 100 μmol/L 5-Fu＋100 μmol/L 5-nitro-2-（3-phenylpropylamino） benzoic acid （5-Fu＋NPPB group）. Changes of the cell volume were monitored and analyzed by the time-lapse imaging technique. Cell apoptotic rates were measured and analysed by Hoechst 33258 staining. Results：The results showed that 5-Fu induced the early cell volume decrease in a few minutes. The cell shrinkage and volume decrease were found after 5-Fu treatment. No significant change was found in cell volume after treatment with 5-Fu＋NPPB. The cell volumes were significantly decreased in 5 time points in 5-Fu group than those of control. The effect of 5-Fu＋NPPB on the cell volume was significantly smaller than that of 5-Fu. The apoptotic rate induced by 5-Fu was （49.2±2.6）% compared with （1.8±0.5）% in control group. The apoptotic rate was decreased to （12.5±2.9） % after treatment with 5-Fu＋NPPB. Conclusion： Chloride channel blockers could inhibit 5-Fu-induced apoptotic cell volume decrease and apoptosis.

关 键 词：氟尿嘧啶 苯甲酸盐类 鼻咽肿瘤 癌 细胞凋亡 氯化物通道 

分 类 号：R73-36[医药卫生—肿瘤]