淋巴瘤凋亡调节基因bcl x mRNA及蛋白的表达  

mRNA and protein expression of apoptosis regulating gene bcl x in lymphomas

作　　者：周晓燕[1] 许良中[1] 何开玲[2] 朱雄增[1] 朱伟萍[1] 张泰明[1] 

机构地区：[1]上海医科大学肿瘤医院病理科,200032 [2]上海医科大学肿瘤医院生物化学教研室,200032

出　　处：《中华病理学杂志》1999年第4期260-263,共4页Chinese Journal of Pathology

基　　金：上海市医学领先专业资助

摘　　要：目的　探讨凋亡调节基因ｂｃｌｘ 在淋巴瘤细胞凋亡调控和肿瘤发生中的作用及在淋巴瘤诊断中的价值。方法　收集３０ 例新鲜标本和１０９ 例存档蜡块（ 均包括淋巴结反应性增生和Ｔ、Ｂ常见类型淋巴瘤） ，用逆转录－ 聚合酶链反应（ＲＴ－ ＰＣＲ） 和免疫组化枸橼酸微波ＡＢＣ法分别观察ｂｃｌｘｍＲＮＡ 和其蛋白的表达及分布。结果　ＲＴ－ ＰＣＲ 显示３０ 例新鲜标本中均有ｂｃｌｘ 的转录，其中２９ 例可见ｂｃｌｘｌ 条带，１３ 例出现ｂｃｌｘｓ 条带，ｂｃｌｘｌ 的强度均强于ｂｃｌｘｓ。免疫组化显示１０９ 例中９７ 例ｂｃｌｘ阳性，呈胞质内细颗粒状，在淋巴结反应性增生中ｂｃｌｘ 呈弱阳性，主要为滤泡中心及滤泡间区细胞阳性，而套细胞为阴性；淋巴瘤细胞常为弥漫强阳性，各类型淋巴瘤染色分数显著高于淋巴结反应性增生（Ｐ＜ ０．０５） ，但各类型淋巴瘤间染色分数和阳性率均无显著差异（Ｐ＞ ０．０５） 。结论　ｂｃｌｘ 在淋巴瘤中呈活跃转录及蛋白高表达，主要以抑制细胞凋亡的ｂｃｌｘｌ 形式存在。ｂｃｌｘ 的蛋白表达特征可能对淋巴结反应性增生和淋巴瘤的鉴别诊断有一定帮助。Objective To observe mRNA and protein expression of bcl x and explore the role of bcl x in the regulation of apoptosis and oncogenesis and their value in differential diagnosis of lymophomas. Methods Reverse transcription polymerase chain reaction (RT PCR) and citric acid microwave ABC immunohistochemistry (ICH) were used to observe mRNA transcription forms and protein distributions of bcl x in 30 frozen tissues and 109 paraffin embedded tissues (both including reactive hyperplasia and common types of lymphoma). Results RT PCR revealed that all 30 frozen cases showed bcl x transcription, of which 29 had bcl x1 band and 13 had bcl xs band. Bcl x1 band was always stronger than bcl xs band. IHC found that 97/109 cases had fine positive bcl x granules in the cytoplasm. In lymphoid reactive hyperplasia, bcl x exhibited weak immunoactivity in cells located in the germinal centers and interfollicular regions of the nodes but not in the mantle zone lymphocytes which surround germinal centers. In lymphomas, bcl x immunoactivity was always strong and diffuse. The staining fractions in different types of lymphomas were significantly higher than those in lymphoid reactive hyperplasias ( P >0.05), but no significant difference was found between the positive rate and staining fraction of different types of lymphomas. Conclusion Bcl x shows active mRNA transcription and high protein expression in lymphomas. The long isoform of bcl x (bcl xl) is the predominant expression. The characteristics of bcl x protein expression may be helpful in differential diagnosis of reactive hyperplasia and lymphoma.

关 键 词：淋巴瘤 细胞凋亡 MRNA 免疫组织化学 BCL-X 

分 类 号：R733.402[医药卫生—肿瘤]