Microarray profiles on age-related genes in the earlier postnatal rat visual cortex  

作　　者：YANG Liu NIE Yu-hong ZHOU Li-hua LIN Shao-chun WU Kai-li 

机构地区：[1]Department of Ophthalmology, Beijing Tiantan Hospital, Capital Medical University, Beijingt00050, China [2]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center , Sun Yat-sen University, Guangzhou, Guangdong 510060, China [3]Department of Anatomy, Zhongshan Medical College , Sun Yat-sen University, Guangzhou, Guangdong 510060, China [4]Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China

出　　处：《Chinese Medical Journal》2011年第10期1545-1550,共6页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （No. 81070765） and the Department of Science ＆ Technology of Guangdong Province, China （No. 2004A30801001）

摘　　要：Background Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that coBackground Accumulating evidence indicates that both innate and adaptive mechanisms are responsible for the postnatal development of the mammalian visual cortex. Most of the studies, including gene expression analysis, were performed on the visual cortex during the critical period; few efforts were made to elucidate the molecular changes in the visual cortex during much earlier postnatal stages. The current study aimed to gain a general insight into the molecular mechanisms in the developmental process of the rat visual cortex using microarray to display the gene expression profiles of the visual cortex on postnatal days.Methods All age-matched Sprague-Dawley rats in various groups including postnatal day 0 （PO, n=20）, day 10 （P10,n=15）, day 20 （P20, n=15） and day 45 （P45, n=10） were sacrificed respectively. Fresh visual cortex from the binocular area （Area 17） was dissected for extraction of total RNA for microarray analyses. Taking advantage of annotation information from the gene ontology and pathway database, the gene expression profiles were systematically and globally analyzed.Results Of the 31 042 gene sequences represented on the rat expression microarray, more than 4000 of the transcripts significantly altered at days 45,20 or 10 compared to day 0. The most obvious alteration of gene expression occurred in the first ten days of the postnatal period and the genomic activities of the visual cortex maintained a high level from birth to day 45. Compared to the gene expression at birth, there were 2630 changed transcripts that shared in three postnatal periods.The up-regulated genes in most signaling pathways were more than those of the down-regulated genes.Conclusions Analyzing gene expression patterns, we provide a detailed insight into the molecular organization of the developing visual cortex in the earlier postnatal rat. The most obvious alteration of gene expression in visual cortex occurred in the first ten days. Our data were a basis to identify new relevant candidate genes that co

关 键 词：microarray analysis earlier postnatal development visual cortex RAT 

分 类 号：H[语言文字]