乳腺癌组织LOX和HIF-1表达及其临床意义的探讨  

Expressions of LOX and HIF-1 protein in breast cancer tissues and their clinical significance

作　　者：蒋奕[1] 杨华伟[1] 唐玮[1] 刘剑仑[1] 

机构地区：[1]广西医科大学附属肿瘤医院乳腺疾病中心,广西南宁530021

出　　处：《中华肿瘤防治杂志》2011年第7期517-520,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广西科学研究与技术开发基金资助(桂科攻0816004-14)

摘　　要：目的:探讨赖氨酰化酶(LOX)在乳腺癌组织、正常乳腺组织和乳腺良性病变组织中的表达,并与乳腺癌患者年龄、肿瘤大小、腋窝淋巴结转移与否、临床分期及HIF-1进行比较,探讨其与乳腺癌侵袭/转移的关系。方法:应用免疫组织化学技术检测56例乳腺癌组织、56例正常乳腺组织及20例乳腺良性病变组织中LOX和HIF-1的表达情况,并应用相关统计学方法进行分析。结果:LOX蛋白在乳腺癌组织、正常乳腺组织和良性病变组织中的表达率分别为48.21%(27/56)、26.78%(15/56)和20.00%(4/20),乳腺癌与正常乳腺组织(P=0.019)、乳腺癌与乳腺良性病变组织(P=0.028)之间的LOX蛋白表达率差异有统计学意义,而正常乳腺组织与良性病变组织之间的LOX蛋白表达率差异无统计学意义,P=0.519;LOX蛋白在不同临床分期(P=0.005)和淋巴结转移(P=0.042)中的表达率差异有统计学意义,但在不同年龄和肿瘤大小患者的癌组织中LOX蛋白的表达率差异无统计学意义,P值分别为0.182和0.058。LOX蛋白与HIF-1α蛋白之间呈正相关,r=0.368,P=0.005。结论:LOX蛋白在乳腺癌组织中高表达。乳腺癌临床分期越晚,肿瘤组织中LOX蛋白的表达率越高。伴淋巴结转移乳腺癌LOX蛋白表达率高于无淋巴结转移者。LOX蛋白表达率与肿瘤大小、患者年龄无关。LOX蛋白与HIF-1α蛋白之间呈正相关。OBJECTIVE： To study the expression of LOX and HIF-1 protein in human breast cancer tissues and explore the relationship between LOX expression and patient＇s age, tumor size, axillary node metastasis, clinical stage, HIF-1 protein expression. METHODS： The expressions of LOX and HIF-1 Protein in 56 cases of human breast cancer tissues, 56 normal breast tissues and 20 cases of breast benign lesion tissues was detected by SP immunohistochemistry, RESULTS： The expression rate of LOX pro tein in breast cancer, normal breast tissues and breast benign lesion tissue were 48. 21 % （27/56）, 26. 78% （15/56） and 20.00% （4/20）, statistical difference was found among them （P= 0.019）. Statistical difference was found between breast carcinoma group, and benign lesion group （P = 0. 028）. And no significant difference was found between normal breast tissues and benign lesion（P= 0.519）. The expression of LOX protein were associated with clinical stage （P= 0.005）, lymph node metastasis （P =0.042）, but had no correlation with age and tumor size （P= 0. 182 and 0. 058）. The LOX protein had significant positive corre- lation with the HIF-1α protein expression in breast cancer （r=0.368,P=0.005）. CONCLUSIONS： LOX protein expression is increased in breast cancer tumor tissues. LOX is related with clinical stage and lymph node metastasis. LOX and HIF-1α have a synergistic effect in breast cancer invasion and metastasis.

关 键 词：乳腺肿瘤 赖氨酰氧化酶 低氧诱导因子-1 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]