胃源性及原发性卵巢癌组织Survivin和VEGF及Smac/DIABLO蛋白表达差异临床意义的研究  

Different expressions of Survivin,VEGF and Smac/DIABLO in metastatic ovarian cancer tissues originated from gastric carcinoma and primary ovarian cancer tissues

作　　者：李勇[1] 孙艳[2] 范立侨[1] 赵群[1] 赵雪峰[1] 郝英杰[1] 

机构地区：[1]河北医科大学第四医院外三科,河北石家庄050011 [2]河北医科大学第三医院妇产科,河北石家庄050051

出　　处：《中华肿瘤防治杂志》2011年第7期528-532,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：河北省普通高校强势特色学科资助项目〔冀教高(2005)52〕;河北省科技厅科研基金资助项目(08276101D-38)

摘　　要：目的:检测胃源性卵巢转移癌及原发性卵巢上皮癌组织Survivin和VEGF及Smac/DIABLO蛋白表达差异,并探讨其意义。方法:采用免疫组织化学和流式细胞术检测Survivin、VEGF和Smac/DIABLO蛋白在胃源性卵巢转移癌、原发性卵巢上皮癌、良性卵巢上皮肿瘤组织和正常卵巢上皮组织中表达,并分析其相关性。结果:与正常和良性疾病组织相比,胃源性卵巢转移癌、原发性卵巢上皮癌组织Survivin、VEGF蛋白表达均明显增强、Smac/DIABLO蛋白表达明显减弱,P值均<0.05;Survivin胞核表达在胃源性卵巢转移癌明显高于原发性卵巢上皮癌,P<0.05。结论:Sur-vivin、VEGF和Smac/DIABLO蛋白共同参与了胃源性卵巢转移癌和原发性卵巢上皮癌进展过程,Survivin细胞核表达差异可能作为两者鉴别的指标。OBJECTIVE：To investigate the different expressions of Survivin, VEGF, Smac/DIABLO and their correlation in metastatic ovarian cancer originated from gastric carcinoma and primary ovarian cancer. METHODS： Immunoehemistry and flow cytometry were used to investigate the expressions of Survivin, VEGF and Smac/DIABLO proteins in normal ovarian epithelium, benign and epithelial ovarian tumor, metastatic ovarian cancer originated from gastric carcinoma, primary epithelial ovarian cancer, and their relationship was analyzed. RESULTS： Expressions of Survivin and VEGF in metastatic ovarian cancer originated from gastric carcinoma and primary epithelial ovar- ian cancer were stronger than those in normal ovarian epithelium, benign and epithelial ovarian tumor, and expression of Smac/DIABLO was weaker（all P〈0.05）. Expression of nuclear Survivin was higher in metastatic ovarian cancer originated from gastric carcinoma than that in primary epithelial ovarian cancer （P〈0. 05）. CONCLUSION： Survivin, VEGF and Smac/DIABLO are important factors involved in growth and metastasis of metastatic ovarian cancer originated from gastric carcinoma and primary epithelial ovarian cancer, and nuclear Survivin may be a biochemical index in differential diagnosis of these two disease.

关 键 词：卵巢肿瘤 淋巴转移 肿瘤标记 生物学 

分 类 号：R737.31[医药卫生—肿瘤]