遗传性非息肉病性结直肠癌血清的蛋白质组学  

Serum proteomic analysis of hereditary nonpolyposis colorectal cancer

作　　者：魏巍[1] 尚秀娟[1] 吕保洲[1] 

机构地区：[1]河南省新乡市中心医院肛肠科,河南省新乡市453000

出　　处：《世界华人消化杂志》2011年第13期1417-1421,共5页World Chinese Journal of Digestology

摘　　要：目的:研究遗传性非息肉病性结直肠癌(HNPCC)与散发性结直肠癌患者术前血清蛋白质的表达差异,以期发现可用于HNPCC诊断的生物学指标.方法:应用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术结合蛋白质芯片分别检测20例HNPCC和25例散发性结直肠癌患者术前血清蛋白质组分.将获得的蛋白质谱采用美国Ciphergen公司的Biomarker Wizard和Biomarker Pattern软件进行分析.结果:以质荷比为7516.68Da和4827.07Da建立的分类决策树模型能将HNPCC和散发性结直肠癌患者区分开来.该模型在测试模式下诊断准确率为86.7%、灵敏度为72.3%、总特异度95.1%、阳性预测值92.5%.盲法验证模式下诊断准确率为75.6%、灵敏度69.8%、总特异度99.2%、阳性预测值100%.结论:利用SELDI-TOF-MS技术建立的HNPCC诊断分类树模型具有较高的敏感度和特异度.AIM：To identify differentially expressed serum proteins between preoperative patients with hereditary nonpolyposis colorectal cancer （HNPCC）and those with sporadic colorectal cancer to find potential markers for the diagnosis of HNPCC.METHODS：The composition of serum proteins was detected by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS）and protein chip assay in 20 preoperative HNPCC patients and 25 patients with sporadic colorectal cancer.Discriminatory serum protein profiles were analyzed with the Biomarker Wizard software and Biomarker Pattern software.RESULTS：Two proteins（m/z 7 516.68 Da and 4 827.07 Da）were chosen to create a decision classification tree model which could discriminate the two groups of patients.In the test mode,the accuracy,sensitivity,specificity,and positive predictive value of the model for prediction of HNPCC was 86.7%,72.3%,95.1%and 92.5%,respectively,while the corresponding values for blinded validation were 75.6%,100%,69.8%and 99.2%,respectively.CONCLUSION：The classification tree model constructed with two differentially expressed proteins identified by SELDI-TOF-MS between HNPCC patients and patients with sporadic colorectal cancer possesses high sensitivity and specificity in the prediction of HNPCC.

关 键 词：遗传性非息肉病性结直肠癌 表面增强激光解吸电离飞行时间质谱 蛋白质组学 

分 类 号：R735.3[医药卫生—肿瘤]