利用高效降解菌株强化修复土壤中DBP及其细菌群落动态解析  

Biodegradation of DBP contaminated soil by high-efficiency degrading strain and dynamics analysis of bacterial community

作　　者：吴学玲[1] 代沁芸[1] 梁任星[1] 王洋洋[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2011年第5期1188-1194,共7页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(30770388)

摘　　要：在实验室条件下,研究土壤中分别加入邻苯二甲酸二丁酯(DBP)和同时加入DBP及高效降解菌JDC13后,对土壤中DBP降解的影响以及土壤中细菌总数和细菌群落结构的动态变化。通过高效液相色谱(HPLC)检测土壤中DBP的残留含量。通过变性梯度凝胶电泳(DGGE)检测土壤细菌群落结构的变化。研究结果表明:只加入DBP的样品A中,DBP在14 d时被完全降解;加入DBP和JDC13的样品B中,DBP在9 d时被完全降解,说明JDC13有效加快了土壤中DBP的降解速度;土壤中细菌总数测定结果显示DBP会抑制土著细菌的生长,但加入JDC13后可以减少DBP的这种不利影响;只加入DBP和同时加入DBP与JDC13对土壤中细菌群落结构的影响十分明显;JDC13、放线菌和β变形菌在土壤DBP降解过程中成为优势种群;大部分优势菌与PAEs(邻苯二甲酸酯)降解菌具有同源性。An experiment was conducted in lab by adding di-n-butyl phthalate （DBP） soil with DBP alone and DBP plus DBP degrader JDC13. Biodegradation of DBP in soil, and its effect of the inoculant JDC13 on soil bacterial population and structure of soil bacterial community were studied. The DBP remaining in soil was determined by HPLC. In Sample A, with the only addition of DBP, the DBP was completely degraded after 14 d. And in Sample B, with the addition of DBP and JDC 13, the DBP in soil was absolutely degraded after 9 d. Changes in the bacterial community were monitored using denaturing gradient gel electrophoresis （DGGE）. The results show that JDC 13 can enhance the biodegradation of DBP in soil. DBP can inhibit the growth of local soil bacteria, but inoculation with JDC13 can reduce the negative effect of DBP on local soil bacteria. DBP alone and DBP plus JDC13 substantially affect the structure of bacterial community in the soil. JDC 13, Actinobacteria and Betaproteobacteria become the dominant bacteria in the DBP-contaminated soil. And most of them are homologous to the Phthalate esters （PAEs） degrader.

关 键 词：DBP生物降解 生物强化 PCR-DGGE 细菌群落 

分 类 号：X172[环境科学与工程—环境科学]