α-2，3-唾液酸糖基转移酶ST3家族分子序列生物信息学分析  

Bioinformatics Analysis on the Molecular Sequence of B-galactoside α-2,3-sialyltransferase Family

作　　者：马汝海[1] 钟连生[2] 潘忠诚[2] 王天骄[2] 何群[2] 

机构地区：[1]中国医科大学基础医学院化学教研室,沈阳110001 [2]中国医科大学基础医学院教育部细胞生物学重点实验室,生物芯片中心,沈阳110001

出　　处：《中国医科大学学报》2011年第6期497-500,511,共5页Journal of China Medical University

基　　金：国家自然科学基金资助项目(20672144)

摘　　要：目的比较探讨人类α-2,3-唾液酸糖基转移酶家族成员间的同源性,探讨其进化规律及其与功能相关性。方法利用网上数据库和生物信息学软件对核酸及蛋白质序列进行同源比较、结构域分析和进化树绘制。结果人类α-2,3-唾液酸糖基转移酶家族一级结构、二级结构均有较高的同源性,系统进化分析显示其各成员来源于同一祖先并在不同时期分野。结论各成员来自于同一祖先的同一家族,其进化方式属于趋异性进化,家族中各亚型底物特异性可能与其糖基化位点相关。Objective To compare, the homology in homo sapiens alph α-2,3-sialyltransferase family and study the patterns of its revolution and its functional correlation. Methods Homology and domain analysis of nucleic acid and protein sequences of beta-galactoside alphα-2,3-sialyltransferase family was conducted by using online data base and bioinformatics software, and phylogenefic tree was constructed. Results The primary and secondary structures of alphα-2,3-sialyltmnsferase family showed high homology. The phylogenetic analysis showed that they were derived from a common ancestor but diverged at different times. Conclusion All members of alphα-2,3-sialyltransferase come from the same family of a common ancestor, and their evolution was in a divergent pattem. The substrate specificity of the family sub- units may be related to the glycosylation sites.

关 键 词：α-2 3-唾液酸糖基转移酶 同源性分析 结构域 系统进化树 

分 类 号：Q53[生物学—生物化学]