脑缺血预处理对神经元神经钙调磷酸酶及磷酸化蛋白激酶B表达的影响  

Effect of cerebral ischemic preconditioning on the expression of calcineurin and phosphorylated protein kinase B in neurons

作　　者：姜永梅[1] 张慧[1] 尹琳[1] 

机构地区：[1]大连医科大学附属第二医院神经内科,116027

出　　处：《中华老年心脑血管病杂志》2011年第6期557-560,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的研究脑缺血预处理(CIPC)对神经元神经钙调磷酸酶(CaN)及磷酸化蛋白激酶B(p-AKT)表达的影响。方法雄性SD大鼠108只,随机分为假手术组、脑缺血组、CIPC组、尼莫地平组、环孢素A组及LY294002组,每组18只。评价各组大鼠神经功能缺损;计算脑梗死体积;检测CaN及p-AKT蛋白表达。结果与脑缺血组比较,CIPC组、尼莫地平组和环孢素A组神经功能缺损评分和脑梗死体积明显降低(P<0.05,P<0.01)。与假手术组比较,其他5组CaN蛋白表达明显升高(P<0.01);与脑缺血组比较,CIPC组、LY294002组CaN蛋白表达降低(P<0.05);与CIPC组比较,尼莫地平组和环孢素A组CaN蛋白表达明显降低(P<0.05,P<0.01)。除LY294002组外,余各组p-AKT蛋白表达与CaN蛋白相反。结论 CIPC降低神经元CaN表达,提高p AKT表达,提供神经保护。尼莫地平和环孢素A增强了神经保护。LY294002抵消了CIPC的保护作用。Objective To study the effect of cerebral ischemic preconditioning（CIPC） on the expression of calcineurin（CaN） and phosphorylated protein kinase B（p-AKT） in hippocampus neu- rons. Methods 108 adult healthy male SD rats were randomly divided into sham operation group, brain ischemia group, CIPC groups, nimodipine group, cyclosporine A group, and LY294002 group. There were 18 rats in each group. The neurological deficit scores were evaluated,the volume of the cerebral infarct was calculated. The expression of CaN and p-AKT at protein level was examined by Western blot. Results The neurological deficit scores and the volume of the cerebral infarct were highest in the ischemia and LY294002 groups,these items in the CIPC group,the ni modipine group and the cyclosporine A group were lower than those in the ischemia group （P 〈 0.05,P 〈 0.01）. The expression of CaN was highest in the ischemia group,it was lower in the CIPC and LY294002 groups than in ischemia group （P 〈 0.05）, and was lowest in the nimodipine and cyclosporine A groups （P 〈 0.05 ,P 〈 0.01）. The expression of p-AKT was lowest in the ischemia and LY294002 groups,and was higher in the CIPC group than in the ischemia group （P 〈 0.05） ;it was highest in the nimodipine and cyclosporine A groups （P 〈 0.05, P 〈 0.01）. Conclusions Continuous ischemia increases the expression of CaN, and reduces the expression of p-AKT. CIPC reduces the expression of CaN, and raises the expression of p-AKT, thereby increasing cerebral ischemic tolerance. Nimodipine and cyclosporine A can reduce the expression of CaN,and increase the expression of p-AKT. LY294002 counteracts the neuroprotection of CIPC.

关 键 词：脑缺血 缺血预处理 神经元 蛋白激酶类 磷酸化酶激酶 尼莫地平 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]