高效液相色谱法检测羊鸡组织中氟苯达唑、噻苯达唑及其代谢物残留量的研究  

Simultaneous Determination of Flubendazole,Thiabendazole and Their Metabolites Residues in Sheep and Chicken Tissues by HPLC

作　　者：李丹[1] 张玉洁[1] 汪霞[1] 应永飞 林仙军 扎依达 贾新建 薛毅[4] 仲锋[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]浙江省兽药监察所,杭州310000 [3]新疆兽药饲料监察所,乌鲁木齐830063 [4]中国动物疫病预防控制中心,北京100026

出　　处：《中国兽药杂志》2011年第6期26-30,共5页Chinese Journal of Veterinary Drug

摘　　要：建立了一种可同时检测羊组织(肌肉、肝脏、肾脏、脂肪)、鸡组织(肌肉、肝脏)中氟苯达唑及代谢物2-氨基氟苯达唑、噻苯达唑及代谢物5-羟基噻苯达唑药物残留的高效液相色谱检测方法。结果表明:氟苯达唑及代谢物2-氨基氟苯达唑、噻苯达唑及代谢物5-羟基噻苯达唑在0.02～1.5μg/mL浓度范围内呈良好的线性关系r,均大于0.999。方法的定量限为20μg/kg。在鸡组织(肌肉、肝脏)中,两种药物及其代谢物在20～800μg/kg的添加浓度范围内,其回收率范围在60%～100%之间;在羊组织(肌肉、肝脏、肾脏、脂肪)中,两种药物及其代谢物在20～200μg/kg的添加浓度范围内,其回收率范围在60%～100%之间;批内变异系数均小于15%、批间变异系数均小于20%。A high performance liquid chromatography（HPLC） method was developed for simultaneous determination of flubendazole（FLUB）,thiabendazole（TBZ） and their metabolites of 2-aminoflubendazole（2-NH2-FLUB）,5-hydroxythiabendazole（5-OH-TBZ）in sheep tissues（muscle,liver,kidney,fat） and chicken tissues（muscle,liver）.A good linearity of the calibration curve was obtained within the range of 0.02~1.5 μg/mL（r0.999）,and the limit of quantification of flubendazole（FLUB）,thiabendazole（TBZ） and their metabolites of 2-aminoflubendazole（2-NH2-FLUB）,5-hydroxythiabendazole（5-OH-TBZ）was 20 μg/kg.The average recoveries of flubendazole（FLUB）,thiabendazole（TBZ） and their metabolites of 2-aminoflubendazole（2-NH2-FLUB）,5-hydroxythiabendazole（5-OH-TBZ）from spiked sheep tissues between 20 and 200 μg/kg ranged from 60% to 100%（n=5） and from spiked chicken tissues between 20 and 800 μg/kg ranged from 60% to 100%（n=5）.Intra-day batch variation coefficients were less than 15% and inter-day batch variation coefficients were both less than 20%.

关 键 词：高效液相色谱法 氟苯达唑 噻苯达唑 代谢物 残留 羊组织 鸡组织 

分 类 号：S859.84[农业科学—临床兽医学]