P53蛋白在喉上皮癌前病变及癌组织中的表达及其意义  

Expression of P53 Protein in Premalignant Lesion and Carcinoma of Larynx

作　　者：周光耀[1] 林代诚[1] 梁传余[1] 张贤良[1] 文定厚[1] 刘亚峰[1] 

机构地区：[1]华西医科大学附属第一医院,成都610041

出　　处：《华西医科大学学报》1999年第3期265-267,共3页Journal of West China University of Medical Sciences

摘　　要：为探讨喉上皮癌变过程中 Ｐ５３ 蛋白表达水平，采用免疫组化技术检测了１１ 例喉上皮单纯性增生（ Ｓ Ｈ Ｅ）、３２ 例非典型性增生（ Ａ Ｈ Ｅ）及 ４２ 例喉鳞状细胞癌（ Ｌ Ｓ Ｃ Ｃ） Ｐ５３ 蛋白的表达。结果： Ｐ５３ 蛋白阳性表达率在 Ｓ Ｈ Ｅ、 Ａ Ｈ Ｅ 及 Ｌ Ｓ Ｃ Ｃ 中分别为 ０００％ 、３１．２５％ 及 ５２．３８％ ；轻、中、重度 Ａ Ｈ Ｅ 阳性率分别为 ０００％ 、３３．３３％ 及７５％ ；高、中、低分化 Ｌ Ｓ Ｃ Ｃ阳性率分别为６２．５０％ 、７６．９２％ 、１５．３９％ ，其差异有高度显著性（ Ｐ＜ ０．０１）。 Ｐ５３ 蛋白表达与喉癌临床分期、分型、颈淋巴转移、局部复发及预后无相关性。结果提示：喉上皮由正常状态向恶性转化的过程中，存在抑癌基因突变产物 Ｐ５３ 蛋白过度表达，可能与喉癌的发生发展有一定关系。To investigate the expression of P53 protein in the premalignant lesion and carcinoma of laryngeal epithelium, the authors used DO 7, an antibody to wild and mutant type P53 protein. Eleven cases of simple hyperplasia of epithelium (SHE), 32 cases of atypical hyperplasia of epithelium (AHE) and 42 cases of laryngeal squamous cell carcinoma (LSCC) were examined by ABC technique. The results showed that the overexpression of P53 protein were 0% , 31.25% and 52.38% in SHE, AHE and LSCC, respectively. The positive rate of P53 protein were 0%, 33.33% and 75.00% in mild, moderate and severe AHE , respectively. In well, moderately and poorly differentiated carcinomas of larynx, the positive rates were 62.50%, 76.92% and 15.39%, respectively. There were highly significant differences( P <0.01). No correlation was found between the P53 protein overexpression and the clinical stages, neck lymph node metastasis, local recurrence and, survival of LSCC. The results suggest that the overexpression of P53 protein may play a role in the pathogenesis and development of LSCC.

关 键 词：喉肿瘤 P53蛋白 喉上皮癌 癌前病变 

分 类 号：R739.650.2[医药卫生—肿瘤]