羟甲基无环鸟苷对携有Ⅰ型单纯疱疹病毒胸苷激酶基因的卵巢癌细胞的体外杀伤作用  

In Vitro Effect of GCV on the Ovarian Cancer Cells Carrying HSV 1-tk gene

作　　者：徐丛剑[1] 张惜阴[1] 金志军[2] 丰有吉[1] 曹斌融[1] 周先荣[1] 许秀兰[3] 顾健人[3] 

机构地区：[1]上海医科大学妇产科医院,200011 [2]第二军医大学长征医院妇产科,上海200003 [3]上海市肿瘤研究所,200032

出　　处：《中国实用妇科与产科杂志》1999年第9期539-541,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金资助

摘　　要：目的　观察羟甲基无环鸟苷(GCV) 对转导携有Ⅰ型单纯疱疹病毒胸苷激酶基因( HSV1 - tk) 的AO、3AO 细胞外杀伤作用。方法　分别在携有HSV1 - tk 基因的AO、3AO 细胞培养液中加入不同浓度的GCV,绘制剂量效应曲线,并以荧光细胞激活分选仪(FACS) 测定凋亡细胞比例及细胞周期比例,以光镜及电镜观察细胞形态变化,以DNA 荧光染色观察细胞核内染色质变化。结果　GCV 在体外对于携有HSV1 - tk 基因的AO、3AO 细胞的半数抑制浓度均介于10 - 1 ～10 - 2μg/ml 之间,用药后S 期细胞比例显著增高。结论　GCV 在体外可高效杀伤携有HSV1 - tk 基因的卵巢癌细胞。Objective\ To observe the in vitro effect of GCV on the HSV 1-tk + ovarian cancer cells.Methods\ HSV 1-tk + AO、3AO cells were cultured in RPMI 1640 containing different concentrations of GCV,and were harvested and numbered 5 days later.The proprtion of apoptosis cell and cell cycle was calculated by FACS.The morphologic changes was observed by light microscopy and electronic microscopy as well,PI staining was also used to determine the DNA changes.Results\ The IC 50 of GCV to the HSV 1-tk + AO、3AO cells are about 10 -1 ～10 -2 μg/ml;After 10μg/ml GCV was added,the ratio of S phase cells was significantly elevated.Conclusions\ GCV could inflict and(or)inhibit HSV 1-tk + ovarian cancer cells with high effect.

关 键 词：卵巢肿瘤 基因治疗 GCV HSV1-TK 

分 类 号：R737.310.5[医药卫生—肿瘤]