牛瑟氏泰勒虫吉林株ITS基因的克隆与系统进化分析  

Cloning and phylogenetic analysis of ITS gene from Theileria sergenti Jilin strain

作　　者：钱年超[1] 张影[1] 薛书江[1] 贾立军[1] 张守发[1] 

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《畜牧与兽医》2011年第6期5-7,共3页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30960278)

摘　　要：本研究根据GenBank上的牛瑟氏泰勒虫内转录间隔区基因(ITS基因)设计合成1对特异性引物,以采自吉林省珲春市某养牛场的血液样本为试验材料,PCR扩增牛瑟氏泰勒虫ITS基因,克隆至pMD-18T Simple载体,进行序列测定,将该基因序列与GenBank上9种已知的泰勒虫相应序列进行比较分析,建立系统进化树。结果表明:牛瑟氏泰勒虫吉林株与美国株亲缘关系较近,其次是水牛泰勒虫,与其他泰勒虫亲缘关系较远。A pair of specific primers was synthesized according to Theileria sergenti internal transcribed spacers （ITS） gene in GenBank. Blood samples were collected from a cattle farm in Hunchun, Jilin province. T. sergenti ITS gene was amplified by PCR and cloned into pMD- 18T Simple vector for sequencing. The result of sequence analysis was compared with those of 9 other Theileria strains available in GenBank and the phylogenetic tree was constructed. The result showed that T. sergenti Jilin strain is closer to American strain, followed by Theileria buffeli, but far from other Theileria strains.

关 键 词：牛瑟氏泰勒虫 ITS基因 克隆 系统发育树 

分 类 号：S852.7[农业科学—基础兽医学]