非小细胞肺癌中VEGF-C、VEGFR-3 mRNA表达对淋巴结转移和预后的研究  

Expression of VEGF-C,VEGFR-3 mRNA and its predictive study on lymph node metastasis and prognosis of non-small cell lung carcinoma

作　　者：亓宪银[1] 李大宏[1] 徐林浩[2] 

机构地区：[1]山东省莱芜市人民医院胸外科,271100 [2]青岛大学医学院附属医院胸外科,山东青岛266021

出　　处：《重庆医学》2011年第15期1478-1480,共3页Chongqing medicine

摘　　要：目的血管内皮生长因子-C(VEGF-C)和血管内皮生长因子受体-3 mRNA是促进恶性肿瘤淋巴管形成的重要因子,通过研究VEGF-C、VEGFR-3 mRNA在非小细胞肺癌中的表达,试图找到肺癌淋巴结转移和预后的规律。方法采用RT-PCR法分析60例新鲜肺癌组织、癌旁正常肺组织及肺门、纵隔淋巴结标本中VEGF-C和VEGFR-3 mRNA的表达,并分析其与临床病理特点之间的关系。结果肺癌组织中VEGF-C mRNA表达阳性率为68.33%(41/60),其中34例VEGFR-3 mRNA阳性,VEGF-C和VEGFR-3的表达密切相关(P<0.01)。VEGF-C mRNA的表达与肺癌的淋巴结转移、分化程度及TNM分期显著相关(P<0.05),但与患者年龄、肿瘤大小及病理类型无关。正常肺组织中未见VEGF-C/VEGFR-3 mRNA表达。结论 VEGF-C/VEGFR-3 mRNA表达与肺癌淋巴结转移及其预后密切相关。Objective Vascular endothelial growth factor C（VEGF-C） and vascular endothelial growth factor receptor 3（VEGFR-3） play important roles in lymphangiogenesis of malignant tumors.This study was designed to investigate the expression of VEGF-C and VEGFR-3 mRNA in non-small cell lung carcinoma,then try to find the rule of lymphatic metastasis and prognosis of lung cancer.Methods The expression of VEGF-C and VEGFR-3 mRNA was examined by RT-PCR in 60 cases of the fresh non-small-cell lung cancer tissues and normal lung tissues,followed by the further analysis on the relationship between VEGF-C/VEGFR-3 mRNA expression and clinical-pathological characters.Results VEGF-C mRNA was detected in 41 of 60（68.33%） lung cancer tissues,and VEGFR-3 mRNA expressed in 34 cases of the VEGF-C mRNA positive tissues.There was a statistically positive correlation between the incidence of VEGF-C and VEGFR-3 mRNA（P0.01）.The expression of VEGF-C mRNA was significantly associated with the lymph node metastasis,differentiation and TNM stages（P0.05）,but not to the patientage,tumor size and pathologic type.The expression of VEGF-C/VEGFR-3 mRNA in normal lung tissues was under detectable level.Conclusion The expression of VEGF-C and VEGFR-3 mRNA are closely related to lymphatic metastasis and prognosis of lung cancer.

关 键 词：肺肿瘤 血管内皮生长因子C 血管内皮生长因子受体3 淋巴结转移 预后 

分 类 号：R734.2[医药卫生—肿瘤]