四膜虫有性生殖过程中特异表达基因TCD3的序列分析  

Sequence Analysis of Conjugation Specific Expressed TCD3 from Tetrahymena thermophila

作　　者：张理光[1] 王清路[1] 朱雅静[1] 王伟[1] 

机构地区：[1]山西大学,生物技术研究所,化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2011年第2期301-306,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(3077029531072000);山西省自然科学基金(2008011063);山西省高等学校优秀青年学术带头人支持计划资助项目

摘　　要：含有CHROMO(chromatin organization modifier)结构域的蛋白参与了染色质结构的调节.本研究从嗜热四膜虫(Tetrahymena thermophila)全基因数据库(www.ciliate.org)和基因表达数据库(tged.ihb.ac.cn)中筛选到有性生殖特异表达TCD3(Tetrahymena chromo domain-containing protein 3)基因.该基因ORF为1 005bp,大核染色体中基因全长1 241bp,含有两个内含子.5′-RACE和3′-RACE表明5′端非编码区(5′-UTR)长度为40bp,3′端非编码区(3′-UTR)长度为90bp.TCD3基因预测编码334个氨基酸构成,含有一个CHROMO结构域.CHROMO结构域聚类分析表明四膜虫Tcd3蛋白可能与线虫CeCDP功能类似,参与异染色质的形成和DNA序列删除.The protein which contains CHROMO（chromatin organization modifier）domain participates the regulation of chromatin structure.Conjugation specific expressed TCD3（Tetrahymena chromo domain-containing protein 3）gene was obtained by bioinformatics from Tetrahymena genome database（www.ciliate.org）.The microarray data showed TCD3 gene was conjugation specific expressed.The ORF of TCD3 contained 1 005 bp,and the macronuclear chromosome counterpart fragment was 1 241 bp including two introns.5′RACE showed 5′untranslated region（5′ UTR）of TCD3 contains 40 bp,3′RACE showed 3′untranslated region（3′ UTR）of TCD3 contains 90 bp.Deduced Tcd3 protein encoded 334 amino acids and included one CHROMO domain.The cluster analysis of CHROMO domain implies Tcd3 might involved in the heterochromatin formation and DNA elimination.

关 键 词：CHROMO结构域 四膜虫 TCD3基因 RACE 聚类分析 

分 类 号：Q786[生物学—分子生物学]