氯胺酮对培养胎鼠皮层神经元NMDA兴奋毒性损伤的保护作用  

Protective effect of ketamine on impairment of cultured primary cortical cells of rat fetus caused by NMDA

作　　者：林也容[1,2] 黄晶[1,2] 李娟[1,2] 林月丽[1,2] 蔡颖谦[3] 郁毅刚[1,4] 

机构地区：[1]解放军第一七五医院 [2]厦门大学附属东南医院妇儿科,福建漳州363000 [3]南方医科大学珠江医院全军神经医学研究所,广东广州510282 [4]厦门大学附属东南医院神经外科,福建漳州363000

出　　处：《临床和实验医学杂志》2011年第11期801-803,808,共4页Journal of Clinical and Experimental Medicine

基　　金：福建省自然科学基金计划项目(2007J0302);国家自然基金面上项目(30672167);全军十一五医学科研基金面上项目(06MB152);福建省漳州市科技计划项目(Z06078)资助;福建省科技计划青年人才项目(2006F3150)

摘　　要：目的研究氯胺酮对培养神经细胞兴奋性氨基酸N-甲基-D-天冬氨酸(NMDA)损伤的保护作用。方法采用原代培养的胎鼠皮层神经细胞模型,给予NMDA直接损伤,加入不同浓度的氯胺酮,测定神经细胞损伤后噻唑蓝(MTT)变化。结果氯胺酮在50～1000μmol/L范围内存在对兴奋性氨基酸损伤的保护作用,非剂量线性相关。结论氯胺酮对神经元NMDA兴奋毒性损伤产生拮抗作用,离体细胞模型中1×102-3μmol/L浓度左右具有保护效果。Objective To study the neuroprotective effect of ketamine for impairment in cultured neurocytes caused by NMDA.Methods By using primary cultured rat cortical neurons,the changes of MTT in neurons impaired by NMDA and protective effects of ketamine were studied.Results The neuroprotective effect of ketamine is closely related to the concentration of ketamine,the concentration between 50～1 000 μmol /L is its effective range and it is not related to linear dosage.Conclusion The neuroprotective effects of ketamine is operated in universality,and 1×102-3 μmol /L L is the best concentration for neuroprotective effect.

关 键 词：大鼠 NMDA 脑损伤 氯胺酮 

分 类 号：R965[医药卫生—药理学]