壳聚糖/聚羟基乙酸移植物桥接大鼠坐骨神经5mm缺损的研究  

The study of rat sciatic nerve regeneration across a 5mm defect bridged by a chitosan/PGA-based nerve graft

作　　者：韦中亚[1] 刘伟[1] 张沛云[1] 顾芸[1] 陈罡[1] 顾晓松[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,江苏226001

出　　处：《交通医学》2011年第2期113-117,共5页Medical Journal of Communications

基　　金：江苏省高校优势建设工程资助项目(PAPD);江苏省自然科学创新攀登项目(BK2008010)

摘　　要：目的:探讨壳聚糖/PGA移植物桥接大鼠坐骨神经5mm缺损后神经再生的进程。方法:雌性SD大鼠24只,体重200±20g,随机分为3组,每组8只。行壳聚糖/PGA移植物桥接大鼠坐骨神经5mm缺损模型,实验分1W,2W,3W 3个时间点,分别于各时间点灌注取材,采用免疫组织化学染色方法观察坐骨神经再生的进程。结果:壳聚糖/PGA移植物桥接大鼠坐骨神经5mm缺损,1W时长入约1 500μm,2W时再生纤维已接近远端桥接处,长入距离约5 000μm,同时远端溃变的碎屑已基本清除;3W时可见部分再生神经纤维已完全通过神经移植物生长至损伤远端,同时可观察到局部有髓鞘蛋白P0的表达。Objective： A novel artificial nerve graft,composed of a chitosan tube and polyglycolic acid（PGA） fiber,was used to bridge a 5mm gap in rat sciatic nerve to investigate its progression of regeneration.Methods： Immunohistochemistry was applied to observe the nerve progression of regeneration after implantation at 1W,2W and 3W with the 24 female SD rats（200±20g 1W： n=8,2W： n=8,3W： n=8）.Results： In the study,at 1 week,the distance of regeneration nerve into graft was about 1 500μm and 5 000μm at 2 weeks;the degeneration debris of the distal nerve stump was basically cleared at 2 weeks;At 3 weeks,the nerve growth passed through the nerve graft to the distal nerve,and meanwhile myelin protein P0 was observed in the local contribution.

关 键 词：坐骨神经 施万细胞 壳聚糖 聚羟基乙酸 周围神经再生 大鼠 

分 类 号：Q819[生物学—生物工程]