Tissue Culture of Lespedeza cyrtobotrya  

短梗胡枝子的组织培养研究(摘要)(英文)

作　　者：陈佳[1,2] 李忠秋[3] 陈晓阳[4] 

机构地区：[1]南京大学金陵学院,江苏南京210089 [2]北京林业大学生物科学与技术学院,北京100083 [3]南京大学生命科学学院,江苏南京210093 [4]华南农业大学林学院,广东广州510642

出　　处：《Agricultural Science & Technology》2011年第3期359-362,共4页农业科学与技术（英文版）

基　　金：Supported by National High Technology Research and Development Program of China (2002AA241111 );Introduction of International Advanced Agricultural Science and Technology Program " 948 "(2001-25)~~

摘　　要：[Objective] The aim was to carry out study on tissue culture of Lespedeza cyrtobotrya. [Method] The seeds of L. cyrtobotrya were used as materials to study on its tissue culture. [Result] The best sterilization time to L. cyrtobotrya seeds was 8 min with 2.1% NaClO,in which shooting percent reached 37.8% and no polluted situations occurred. In the primary culture with the MS as basal medium,the concentration of 6-BA showed a significant effect on the index of buds differentiation,the optimum differentiation culture medium was MS＋BA 1.0 mg/L＋NAA 0.1 mg/L＋2,4-D 0.01 mg/L,on which the index of generation could reach 6.69. The optimum subculture medium was MS＋6-BA 1.0 mg/L＋2,4-D 0.05 mg/L. The plants can generate the highest roots and rooting percent with IBA 0.50 mg/L. [Conclusion] This study had provided theoretical basis for genetic improvement of L. cyrtobotrya.[目的]研究短梗胡枝子(Lespedeza cyrtobotrya)的组织培养。[方法]以短梗胡枝子种子为材料,对其组织培养进行了系统研究。[结果]2.1%次氯酸钠灭菌8min,发芽率较高,且污染率为0;在初代培养中,基础培养基为MS,6-BA对分化系数影响达到显著水平,适宜的增殖培养基为MS+1.00mg/L6-BA+0.10mg/LNAA+0.01mg/L2,4-D,分化系数可达6.69;在继代培养中,适宜的培养基为MS+1.00mg/L6-BA+0.05mg/L2,4-D;在生根培养中,IBA浓度为0.50mg/L时,生根率和平均生根数都最佳。[结论]为进一步开展胡枝子基因工程遗传改良提供了理论依据。

关 键 词：Lespedeza cyrtobotrya Tissue culture REGENERATION 

分 类 号：S541.5[农业科学—作物学]