表皮生长因子受体、人类表皮生长因子2、拓扑异构酶Ⅱ在食管上皮癌变过程中的表达及意义  

Expression and significance of EGFR, Her-2 and TOPO H in esophageal canceration course

作　　者：姚苏霞[1] 李丽[2] 杨宣琴[2] 孙瑞芳[2] 王全红[2] 王晓菊[2] 

机构地区：[1]山西医科大学研究生院,030001 [2]山西省肿瘤医院病理科

出　　处：《肿瘤研究与临床》2011年第5期303-306,共4页Cancer Research and Clinic

摘　　要：目的探讨表皮生长因子受体（EGFR），人类表皮生长因子2（Her－2），拓扑异构酶Ⅱ（TOPOⅡ）在食管上皮癌变过程中的蛋白表达情况与临床病理参数的关系以及三者之间的相互关系。方法收集107例食管鳞状细胞癌标本，均包括食管正常上皮、上皮内瘤变和鳞状细胞癌。应用组织芯片技术和免疫组织化学En Vision二步法分析EGFR、Her－2、TOPOⅡ的蛋白表达。结果从食管正常鳞状上皮、低级别上皮内瘤变、高级别上皮内瘤变到癌的发展过程中EGFR与TOPOⅡ表达率呈现逐步上升的趋势（8．41％、7．94％、27．27％、50．47％；3．74％、4．76％、20．45％、43．93％）（P=0．031）。且随着组织学分级的增加二者的表达水平递增（P=0．009），但与性别、年龄、浸润深度、淋巴结转移情况无相关关系。Spearman相关分析显示其二者的表达呈正相关（r1＝0．410，P〈0．05）。Her－2蛋白表达在3组之间差异无统计学意义（P〉0．05），与各病理学参数比较差异亦无统计学意义（P〉0．05），且与EGFR、TOPOⅡ蛋白表达无相关性（rf0．112，P〉0．05；r，=0．186，P〉0，05）。结论EGFR、TOPOⅡ与食管鳞状细胞癌发生、发展密切相关，尤其在高级别上皮内瘤变阶段其蛋白表达发生质的改变。而Her-2基因在山西地区食管癌发生、发展中作用尚不确定。Objective To investigate the expression of EGFR, Her-2 and TOPOⅡ in esophageal eaneeration course, analyze the correlation between the expression and clinical pathological parameters and the correlation of the three genes. Methods EGFR, Her-2 and TOPO Ⅱ were detected by Tissue microarray technology and Envision immunohistochemistry method in 107 cases of esophageal carcinoma, including normal esophageal epithelium, esophageal intraepithelial ncoplasia and esophageal squamous cell carcinoma. Results The positive expression rates of EGFR and TOPO Ⅱ display an improving trend from normal esophageal epithelium, intraepithelial neoplasia to carcinoma （P =0.031） in the above four groups. The positive expression rates of EGFR were 8.41%, 7.94 %, 27.27 %, 50.47 %, and TOPO Ⅱ were 3.74 %, 4.76 %, 20.45 %, 43.93 %. Furthermore, the expression showed gradually incresing with histological grades advance （P =0.009）. There was no correlation between EGFR or TOPO Ⅲ and gender, age, lymph node or distant metastasis （P 〉0.05）. There was a positive correlation between EGFR and TOPO Ⅱ （r1=0.410, P 〈0.05）. There were no significance of Her-2 protein expression among the three groups（P 〉0.05）, no correlation was obtained between Her-2 and gender, age, the depth of invasion or lymph node metastasis （P 〉0.05）, no relationship between Her-2 and EGFR or TOPO Ⅱ either. Conclusion EGFR and TOPO Ⅱ are closely related to the occurrence and development of esophageal squamous cell carcinoma, their expressions all make a qualitative change in the esophageal high-grade intraepithelial neoplasia. The role of Her-2 in the development of esophageal squamous cell carcinoma is still not definite in Shanxi province.

关 键 词：食管肿瘤 受体 表皮生长因子 人类表皮生长因子2 拓扑异构酶Ⅱ 

分 类 号：R735.1[医药卫生—肿瘤]