应用PCR-SSCP-EB染色法检测卵巢上皮性肿瘤P16基因突变  

Detecting the mutation of p16 gene in Ovarian epithelial tumor by PCR?SSCP.

作　　者：潘福娟[1] 宋晓凯[2] 崔大祥[3] 王彩芹[4] 郑奇[1] 崔吉[1] 

机构地区：[1]吉林市中心医院,132011 [2]吉林医学院 [3]第四军医大学全军基因诊断技术应用研究所 [4]吉林医学院附属医院

出　　处：《中国优生与遗传杂志》1999年第5期23-23,43,共2页Chinese Journal of Birth Health & Heredity

摘　　要：１６ 基因是一种新发现的肿瘤抑癌基因，已在人类许多肿瘤中检测出Ｐ１６ 基因突变。我们应用ＰＣＲＳＳＣＰＥＢ染色技术，从石蜡包埋卵巢上皮性肿瘤组织切片中检测Ｐ１６ 基因第二外显子，结果显示Ｐ１６ 基因突变率为５３．５７％ ，表明Ｐ１６基因与卵巢上皮性肿瘤发生密切相关。p16 gene is a neuly found tumor suppressor gene,The mutation of it has been detected in many human tumors.Zn this study we used polymerase chain reaction?(PCR)?single strand conformation polymorphism (SSCP)?EB staining to detect the 2nd exon of p16 gene in paraffin embedded tissues of ovarian epithelial tunor.The mutation rate was 53.57%.The results suggested that the anomaly of p16 gene is closely related to the ovarian epithelial tumor

关 键 词：卵巢肿瘤 基因 P16突变 聚合酶链反应 

分 类 号：R737.310.2[医药卫生—肿瘤]