匹罗卡品癫痫模型中蛋白质netrin-1的表达与苔藓纤维出芽的动态变化  

The expression of netrin-1 and the process of mossy fiber sprouting after pilocarpine-induced epilepsy model in rats

作　　者：郑佳丽[1] 杨金升[1] 石向群[1] 马亚杰[1] 刘学娟[1] 

机构地区：[1]兰州军区兰州总医院神经内科,甘肃兰州730050

出　　处：《癫痫与神经电生理学杂志》2011年第3期129-131,共3页Journal of Epileptology and Electroneurophysiology(China)

摘　　要：目的：探讨神经诱向因子蛋白质netrin-1在癫痫持续状态后海马苔藓纤维出芽（MFS）中的作用。方法：氯化锂-匹罗卡品建立大鼠颞叶癫痫（TLE）模型，采用Timm染色和免疫组化的方法分别检测MFS和netrin-1在大鼠海马组织中的表达。结果：TLE组大鼠在模型形成的第2周和第4周，海马齿状回内netrin-1的表达较正常对照组明显增加，并可见到MFS，穿越齿状回颗粒细胞层到达内分子层，并形成一条致密的层状带。结论：癫痫状态后在海马齿状回netrin-1的表达上调，证明其可能参与了癫痫后MFS过程。Objective：To explore the correlation between axon guiding cues netrin-1 and the process of aberrant mossy fiber sprouting （MFS） following status epilepticus（SE）in rats. Methods：The temporal lobe epilepsy（TLE）animal model was established by lithium-pilocarpine in rats. The process of aberrant MFS and the expression of netrin-1 was observed with Timm and immunohistochemical staining. Results： In the 2nd and 4th weeks after seizure,netrin-1 immunostaining showed an increase of netrin-1 in the dentate gyrus and MFS was found after TLE. Conclusion：The netrin-1 may be involved in the process of MFS.

关 键 词：蛋白质netrin-1 颞叶癫痫(TLE) 苔藓纤维出芽(MFS) 

分 类 号：R742.1[医药卫生—神经病学与精神病学]