罗布麻叶黄酮类化合物的分离提取及对体外皮质酮损伤的保护作用评价  

Separation flavonoids and study of the protection of the cultured pc-12 cell lesioned by corticoeron from Folium Apocyni Veneti

作　　者：吴桂梅[1] 刘春明[1] 张语迟[1] 郑梅竹[1] 李赛男[1] 

机构地区：[1]长春师范学院中心实验室,长春130032

出　　处：《药物分析杂志》2011年第6期1012-1015,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(30970299);吉林省科技厅重点项目(20090936)

摘　　要：目的:应用高速逆流色谱分离提取罗布麻叶中的黄酮类化合物,并对所分离的成分进行定性和定量检测及抗抑郁活性评价。方法:以溶剂配比为乙酸乙酯-乙腈-水-醋酸(5∶0.8∶5∶0.02)作为制备型逆流色谱分离的溶剂系统,并利用高效液相色谱对所分离的成分进行定性和定量检测,用pc-12细胞进行抗抑郁活性评价。结果:应用高速逆流色谱技术一次性分离得到4个黄酮类单体化合物,分别为白麻苷、乙酰化金丝桃苷、三叶豆苷和紫云英苷,经高效液相色谱检测其纯度均达到95%,同时得到2个混合物组分,分别为金丝桃苷/异槲皮素组和槲皮素/山柰酚组。罗布麻叶单体黄酮及粗提物均具有抗抑郁活性。结论:应用高速逆流色谱分离单体化合物具有方法简单,方便快捷的特点,所分离的单体化合物抗抑郁活性较强,具备进一步开发的价值,且各单体化合物及组分的抗抑郁活性有一定的规律。Objective：Separation of the flavonoids from natural plant of Folium Apocyni Veneti by high speed counter current chromatography（HSCCC） was established.The qualitative and quantitative measurement and the protection on the cultured pc-12 cell lesioned by corticoeron of the constituents were also taken.Method：Solvent system composing of ethyl acetate-acetonitrile-water-acetic acid by the volumn ratio of 5∶0.8∶5∶0.02 was used for preparative HSCCC,the qualitative and quantitative measurement were determined by high performance liquid chromatography（HPLC）,and the protection on the cultured pc-12 cell lesioned by corticoeron was evaluated.Results：Four individual compounds composed of quercetin-3-O-malatoside,acetyled hyperoside,and astragaline with the purity above than 95% and two mixtures composed of isoquercetin/hyperoside and kaempferol/quercetin were separated via one-step HSCCC separation.The experiments demonstrated that crude extract and individual compounds being separated had obvious protection on the cultured pc-12 cell lesioned by corticoeron.Conclusion：The HSCCC method provided a simple and effective tool for the separation of the compounds from natural plants.The four flavonoids and two mixtures had the obviously protective effect on the cultured pc-12 cell lesioned by corticosteron and showed a reasonable,should be researched further more.

关 键 词：高速逆流色谱 罗布麻叶 PC-12细胞 抗抑郁活性 评价 

分 类 号：R917[医药卫生—药物分析学]