p16、CyclinD_1和CDK4基因在胰腺癌中的表达及其意义  

THE SIGNIFICANCE OF EXPRESSION OF P16、CYCLIN D_1 AND CDK4 IN PANCREATIC CARCINOMA

作　　者：何劲松[1] 赖焕辉[1] 姜立华[1] 吕新生[2] 曹亚[3] 廖伟[3] 黄幸青[1] 王江桥[1] 郭建平[1] 

机构地区：[1]广东省粤北人民医院普外科,韶关512026 [2]湖南医科大学湘雅医院普外科 [3]湖南医科大学肿瘤研究所

出　　处：《中国普外基础与临床杂志》1999年第6期337-338,共2页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：为探讨抑癌基因ｐ１６ 、癌基因Ｃｙｃｌｉｎ Ｄ１ 和ＣＤＫ４ 在胰腺癌中的作用及其相互关系，应用免疫组织化学方法检测了ｐ１６ 、Ｃｙｃｌｉｎ Ｄ１ 和ＣＤＫ４ 基因在胰腺癌中的表达。结果：ｐ１６ 在胰腺癌中为低表达，Ｃｙｃｌｉｎ Ｄ１ 和ＣＤＫ４ 呈过度表达；ｐ１６ 与Ｃｙｃｌｉｎ Ｄ１ 呈负相关关系（ Ｐ＜ ０ ．０５） ，Ｃｙｃｌｉｎ Ｄ１ 与ＣＤＫ４ 呈正相关关系（ Ｐ＜ ０ ．０５） 。提示胰腺癌发生机理涉及ｐ１６ 、Ｃｙｃｌｉｎ Ｄ１ 和ＣＤＫ４ 基因的异常，ｐ１６ 低表达和Ｃｙｃｌｉｎ Ｄ１ 高表达在胰腺癌发生中起协同作用。To study the mechanism of p16,Cyclin D 1 and CDK4 and their relationship with pancreatic carcinoma, their expressions were examined by immunchistochemistry methods. Results: overpression of Cyclin D 1 and CDK4 was revealed in these samples and p16 was undertectable. There was a negitive correlation between p16 and Cyclin D 1 ( P <0.05), and a positive relation between Cyclin D 1 and CDK4 ( P <0.05). The results indicate that abnormality of p16, Cyclin D 1 and CDK4 may be involved in the molecular mechanism of pancreatic carcinoma, p16 lower expression and Cyclin D 1 over expression may coexit in the development of pancreatic carcinoma.

关 键 词：基因表达 胰腺癌 免疫组化 P16 CYCLIND1 CDK4 

分 类 号：R735.902[医药卫生—肿瘤]