赤芍中芍药苷、芍药内酯苷双指标考察赤芍总苷提纯工艺优化  

Process Optimization for Simultaneous Extraction and Purification of Paeoniflorin and Albiflorin from Radix Paeoniae Rubra

作　　者：陈立江[1] 段洪云[1] 张胜[2] 朱鹏飞[3] 刘宇[1] 王程程[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036 [2]辽宁省药物研究院,辽宁沈阳110015 [3]首都医科大学中医药学院,北京100069

出　　处：《食品科学》2011年第12期96-100,共5页Food Science

基　　金：辽宁省自然科学基金项目(20092015);沈阳市科学技术计划项目(F10-205-1-28);辽宁大学"211工程"三期建设项目

摘　　要：目的:双指标考察赤芍的提取纯化工艺。方法:以芍药苷和芍药内酯苷为指标,采用正交试验设计,确定了赤芍的最佳提取工艺,采用大孔吸附树脂纯化赤芍提取物。结果:赤芍的最佳提取工艺为:70%乙醇用量为8mL/g、回流提取3次,每次2h。最佳纯化工艺为:选用AB-8型大孔吸附树脂,径高比为1:8,最大上样量为4.4BV,水洗量为3BV,洗脱流速为1.5BV/h,洗脱剂为40%乙醇,洗脱剂用量为6BV。结论:优选出的提取纯化工艺简便、实用,得到的赤芍总苷纯度高。Objective：To optimize the simultaneous extraction and purification of paeoniflorin and albiflorin from Radix Paeoniae Rubra.Methods： The optimal extraction conditions for achieving the highest total yield of paeoniflorin and albiflorin were determined using orthogonal array design.Further,the purification of paeoniflorin-albiflorin extract by microporous resin adsorption was explored.Results： The optimal extraction conditions were 70% ethanol as extraction solvent at a material-to-liquid ratio of 1：8（g/mL） for reflux extraction repeated three times for 2 h each time.The optimal purification was achieved on AB-8 macroporous adsorption resin column with a diameter/height ratio of 1：8 under the following conditions： sample loading amount of 4.4 BV,washing with 3 BV water and eluting with 6 BV 40% ethanol at a flow rate of 1.5 BV/h.Conclusion： The optimized extraction process is simple and practical and can provide high purity of total paeony glycoside（TPG,mainly paeoniflorin and albiflorin）.

关 键 词：赤芍 提取 纯化 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]