胶体金粒径对氯霉素胶体金试纸条性能的影响  

Effect of Colloidal Gold Particle Size on the Performance of Chloramphenicol Test Strips

作　　者：罗舜菁[1] 陈发荣[1] 刘成梅[1] 邹常春[2] 邱声艳 洪太凤 高鹏[1] 陈婷婷[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学资产处,江西南昌330031 [3]瑞昌人民医院,江西瑞昌332200 [4]School of Applied Sciences,Temasck Polytechnic

出　　处：《食品科学》2011年第12期120-124,共5页Food Science

基　　金：江西省教育厅科学技术研究项目(GJJ09062)

摘　　要：采用柠檬酸三钠法分别制备出粒径为20、30、40nm的3种胶体金溶液,并标记抗氯霉素(CAP)单克隆抗体,另分别制备出竞争法氯霉素胶体金试纸条,并且对各种粒径胶体金制备试纸条的工艺进行优化,比较各种试纸条的性能。结果发现,利用30nm的胶体金制得的试纸条检测限(消线)为3ng/mL,检测快速、稳定,而20、40nm的胶体金制得的试纸条检测限(消线)分别为7、9ng/mL,稳定性较差。30nm的胶体金制备的胶体金试纸条在稳定性、灵敏度方面都明显优于20nm和40nm。Colloidal gold particles with three different particle sizes of 20,30 nm and 40 nm were prepared by the sodium citrate method.The colloidal gold particles with various particle sizes were then used to prepare test strips with colloidal gold-labeled anti-chloramphenicol（CAP） monoclonal antibodies.The preparation parameters of these test strips were optimized and their performance was compared.The test strip containing 30 nm colloidal gold particles exhibited a detection limit of 3 ng/mL.However,the detection limits of the test strips containing colloidal gold particles of 20 nm and 40 nm were only 7 ng/mL and 9 ng/mL,respectively.Moreover,the test strip containing 30 nm colloidal gold particles was more stable and sensitive than the other two and could therefore provide a more suitable marker for testing chloramphenicol.

关 键 词：氯霉素 胶体金 粒径 试纸条 

分 类 号：R446.61[医药卫生—诊断学]