ELAC2对前列腺癌细胞Du-145生长影响的体内外实验研究  

Effect of ELAC2 on Growth of Human Prostate Cancer Du-145 Cells in vitro and in vivo

作　　者：宁萍[1] 焦旸[1] 胡旭东[1] 朱巍[1] 徐加英[1] 薛景[2] 樊赛军[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室,江苏苏州215123 [2]苏州市立医院东区放疗科,江苏苏州215001

出　　处：《苏州大学学报（医学版）》2011年第2期179-182,249,共5页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30701001);长江学者和创新团队发展计划资助项目(IRT0849);苏州大学高校省级重点实验室开放课题资助项目(KJS1029)

摘　　要：目的研究ELAC2在体内外对前列腺癌生长的影响。方法利用脂质体转染法将ELAC2基因转入Du-145细胞,建立稳定表达ELAC2的细胞株,同时设立未转染Du-145和转染空载体的Du-145/pcDNA3作为对照。采用RT-PCR和Western blot法检测转染前后ELAC2的mRNA和蛋白表达水平;MTT法检测细胞生长和增殖情况。采用BALB/c裸鼠建立Du-145前列腺癌移植瘤动物模型,检测在体内ELAC2对前列腺癌生长的影响。结果 RT-PCR检测结果显示,ELAC2在Du-145/ELAC2细胞中mRNA表达明显增加,Western blot法检测同样发现ELAC2蛋白表达水平显著升高。MTT检测结果表明,Du-145/ELAC2细胞增殖较对照组细胞明显减缓(P<0.05)。在体内,Du-145/ELAC2组平均瘤质量和瘤体积均较对照组明显降低(P<0.05)。结论提高ELAC2基因表达水平能明显抑制体内外前列腺癌细胞Du-145的生长,提示ELAC2可作为前列腺癌治疗的一个新基因。Objective To identify the effect of ELAC2 on the growth of human prostate cancer Du-145 cells in vitro and in vivo.Methods ELAC2 was stable transfected into prostate cancer Du-145 cells by Lipofectamine transfection assay,while Du-145 cells transfected with/without empty vector pcDNA3.0 were also developed as the negative controls.The mRNA and protein expression of ELAC2 were detected by RT-PCR and Western blot assay.Cell proliferation was assessed by MTT assay.The allograft prostate tumor model was developed in BALB/c nude mice,which were subcutaneously inoculated with Du-145/ELAC2 cells and the negative control cells,and was used to detect the effects of ELAC2 on tumor growth in vivo.Results As determinated by RT-PCR and Western blot assay,mRNA and protein expression of ELAC2 were significantly increased in Du-145/ELAC2 cells.Cell proliferation of Du-145/ELAC2 was obviously decreased compared with that of negative control cells（P0.05）.Tumor size of Du-145/ELAC2 was obviously smaller than the control tumor size（P0.05）.Conclusion Overexpression of ELAC2 significantly inhibits proliferation of human prostate cancer Du-145 cells in vitro and in vivo,which suggests that ELAC2 may be a novel target for prostate cancer therapy.

关 键 词：ELAC2基因 前列腺癌 细胞生长 稳定转染 裸鼠 

分 类 号：R737.25[医药卫生—肿瘤]