甜荞和苦荞品种遗传多样性的RAPD分析  

RAPD Analysis for Genetic Diversity of Nineteen Common and Tartary Buckwheat Varieties

作　　者：邓琳琼[1,2] 张奎[1] 黄凯丰[1] 陈庆富[1] 

机构地区：[1]贵州师范大学生命科学学院植物遗传育种研究所,贵州贵阳550001 [2]毕节学院岩溶山区生态建设研究所,贵州毕节551700

出　　处：《安徽农业科学》2011年第15期8895-8898,共4页Journal of Anhui Agricultural Sciences

基　　金：燕麦荞麦国家"十二.五"现代农业产业体系荞麦育种岗位科学家资金项目;贵州省动植物育种专项[黔农育专字(2010)023号];国家自然科学基金项目(31060207);贵州省农业攻关项目[黔科合NY字(2010)3094]

摘　　要：[目的]对甜荞和苦荞品种遗传多样性进行RAPD分析。[方法]以7个随机引物对贵州省1999～2010年荞麦区甜荞和苦荞参试品种及其亲本等合计19个品种进行RAPD分析。[结果]共获得149条DNA扩增带,其中多态性谱带141条,多态性谱带的平均比率为94.89%。多态性分析及聚类分析表明,供试品种彼此均有一定的差异,其中威宁甜荞品种彼此亲缘关系较近,而其他甜荞品种间关系较远。遗传变异性程度以种间差异最大,其次是甜荞种内不同品种间,而苦荞种内不同品种间的遗传变异性最小。[结论]研究初步建立了19个品种的RAPD指纹图谱。[Objective] The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA（RAPD）.[Result] A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion] The RAPD fingerprints of the 19 buckwheat varieties were established in present study.

关 键 词：甜荞 苦荞 RAPD 遗传多样性 

分 类 号：S132[农业科学—农业基础科学]