高效萃取体系RP-HPLC测定食品中的对位红和苏丹红  

Determination of Para Red and Sudan Red Dyes in Food by Reversed Phase High Performance Liquid Chromatography

作　　者：彭碧宁 梁玉英 冯家望 丘琼 陈晏 

机构地区：[1]珠海出入境检验验疫局技术中心,广东珠海519015

出　　处：《现代食品科技》2011年第6期715-718,共4页Modern Food Science and Technology

摘　　要：本文研究使用高效萃取体系，建立反相高效液相色谱（RP-HPLC）同时测定食品中对位红、苏丹红Ⅰ～Ⅳ号及苏丹红7B。样品用甲醇-乙腈-丙酮萃取液提取，采用聚硅烷C18色谱柱（Shiseido C18 150mm×4．6mmi．d．，5μm）分离以水-乙腈为流动相，流速1．0mL／min，等度洗脱；用紫外一可见光二极管阵列检测器测定，检测波长为500nm。研究结果表明该方法对苏丹红、对位红的最低检测限为0．1mg／kg，标准曲线的线性相关系数线（r=0．9998），在0．5-5．0μg／mL添加水平，加标回收率为85％-~106％，RSD为2．14％0．82％。该方法涉及的化学试剂少，设备少，操作简便，结果准确，是食品中对位红、苏丹红类染料的快速有效检测方法。A method was developed for the determination of Para Red and Sudan dyes in food by reversed phase-high performance liquid chromatography （RP-HPLC） with a new simple extract system. Sample was extracted from food with methanol-acetonitrile-acetone. The analysis was performed on a Shiseido C18 column（150 mm×4.6mm i.d., 5μm）, with water-acetonitrile as the mobile phase at a flow rate of 1.0mL/min. Detection was carded out at the wavelength of 500nm and confirmed by spectra. All of the six compounds demonstrated good linear relationship （r=0.9998）. The limit of detection （LOD） （spiked at the levels of 0.5-5.0 μg/mL） was 0.1 mg/kg. The average recoveries for all six dyes were ranged from 85%-106%, and the relative standard deviations were form 2.14%.-4.82%. The method is sensitive, reliable and can be applied for the analysis of Para Red and Sudan dyes in food.

关 键 词：高效萃取体系 反相高效液相色谱 对位红 苏丹红 

分 类 号：TS207.3[轻工技术与工程—食品科学]