酵母金属硫蛋白对g12细胞抗γ射线损伤的作用  

EFFECT OF SACCHAROMYCES CEREVISIAE BD101Cu--METALLOTHIONEIN ON RESISTINGIRRADIATION DAMAGE IN G12 CELLS

作　　者：薛宏伟[1] 林慰慈[1] 安丹 舒建芬[2] 刘素萍[2] 林稚兰[2] 

机构地区：[1]北京医科大学公共卫生学院,北京100083 [2]北京大学生命科学院,北京100871

出　　处：《菌物系统》1999年第4期405-408,共4页Mycosystema

基　　金：国家自然科学基金!39737021

摘　　要：用胞质阻断法微核试验和单细胞凝胶电泳法检测酵母金属硫蛋白（ＢＤ１０１Ｃｕ－ＭＴ）对(6０)Ｃｏ－γ射线诱发的ｇ１２细胞微核形成和ＤＮＡ链断裂的影响。结果表明１０和５０μｇ／ｍｌＢＤ１０１Ｃｕ－ＭＴ处理，对１或３Ｇｙγ射线诱发的双核微核细胞率和１Ｇｙγ射线诱发的彗星细胞频率及核ＤＮＡ迁移距离的增加有明显的抑制作用．提示酵母金属硫蛋白对γ射线诱发ｇ１２细胞的遗传损伤有拮抗作用．The effects of Cu-methllothionein from Saccharomyces cerevisiae(BD101 Cu-MT) on micronucleus formation and DNA sbrand breaks in g12 cellsinduced by 60Co r ray were investigated using cytochalasin B--blocked method andsingle cell electrophoresis assay. The results have shown that the trentment of 10 and50ug /mL BD101 Cu--MT could decrease the ratio of micromucleated binucleated cellsinduced by 1 or 3 Gy r--ray and inhibit the increase in frequency of comet cells anddistance of migration of nucleur DNA induced by 1 Gy r--ray. R suggest that there isa antagonistic effect of BD101 Cu-MT on genetic damage in g12 cells induced by y--ray.

关 键 词：酵母 金属硫蛋白 Γ射线 g12细胞 

分 类 号：R818.053[医药卫生—放射医学] Q949.326.1[医药卫生—临床医学]